FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491812

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491812
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77509
Sequences flagged as poor quality0
Sequence length301
%GC51

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA44725.769652556477312No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA30083.880839644428389No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA29783.8421344618044353No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA24693.1854365299513603No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA24053.1028654736869266No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA19932.571314298984634No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA16692.153298326645938No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA16472.1249145260550386No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA16362.110722625759589No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA16312.1042717619889304No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA14461.8655898024745512No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA13181.7004476899456837No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA12931.6681933710923893No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA12251.5804616238114282No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA11031.4230605478073513No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA10761.3882258834457932No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA9731.2553380897702202No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA9351.2063115251132126No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA8961.1559947877020733No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA8891.1469635784231509No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA8841.140512714652492No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA8341.076004076945903No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA7450.9611787018281748No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA7250.9353752467455392No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA7180.9263440374666168No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA6660.8592550542517643No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6620.8540943632352372No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6370.8218400443819428No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA6350.8192596988736792No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5880.7586215794294856No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5610.7237869150679276No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5350.6902424234605014No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5250.6773406959191836No Hit
GGACTACAAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA5200.6708898321485247No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA4920.6347649950328349No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4850.6257337857539125No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4560.5883187758840909No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4560.5883187758840909No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC4240.5470332477518739No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4180.5392922112270833No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4060.523810138177502No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4050.5225199654233702No Hit
GGACTACAAGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA3980.5134887561444477No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3620.4670425369957037No Hit
GGACTACTAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA3620.4670425369957037No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA3590.4631720187333084No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3480.44898011843785884No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA3360.43349804538827746No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA3180.4102749358139055No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3100.39995355378085123No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2790.3599581984027661No Hit
GGACTACACGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA2790.3599581984027661No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2770.3573778528945026No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2760.3560876801403708No Hit
GGACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA2630.3393154343366577No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2480.319962843024681No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA2420.31222180649989034No Hit
GGACTACAAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGCCTCA2320.29932007895857254No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA2260.2915790424337819No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2240.28899869692551833No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2220.28641835141725475No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2160.2786773148924641No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA2150.2773871421383323No Hit
GGACTACTAGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA2130.2748067966300688No Hit
GGACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2100.2709362783676734No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA2000.2580345508263557No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1980.2554542053180921No Hit
GGACTACTCGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA1970.25416403256396036No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1860.23997213226851075No Hit
GGACTACACGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA1740.22449005921892942No Hit
GGACTACCCGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA1730.22319988646479763No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1730.22319988646479763No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1620.20900798616934807No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1610.2077178134152163No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1570.20255712239868917No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1560.20126694964455744No Hit
GGACTACCAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA1540.19868660413629385No Hit
GGACTACAAGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGTGCCTCA1480.1909455676115032No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1480.1909455676115032No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1460.1883652221032396No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1440.18578487659497606No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1430.1844947038408443No Hit
GGACTACTAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGCCTCA1420.1832045310867125No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1420.1832045310867125No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1400.18062418557844895No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1390.17933401282431718No Hit
GGACTACTGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA1370.1767536673160536No Hit
GGACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1300.16772245803713118No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1270.16385193977473583No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1270.16385193977473583No Hit
GGACTACACGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGCCTCA1270.16385193977473583No Hit
GGACTACTCGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA1250.16127159426647228No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1220.15740107600407696No Hit
GGACTACCCGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA1220.15740107600407696No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1210.15611090324994517No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1190.1535305577416816No Hit
GGACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1180.15224038498754983No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1160.14966003947928627No Hit
GGACTACAGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA1130.14578952121689095No Hit
GGACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1120.14449934846275916No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1110.14320917570862737No Hit
GGACTACCCGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1100.1419190029544956No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1090.14062883020036385No Hit
GGACTACACGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGTGCCTCA1050.1354681391838367No Hit
GGACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1050.1354681391838367No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1030.13288779367557316No Hit
GGACTACCAGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA1030.13288779367557316No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1020.13159762092144137No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1020.13159762092144137No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1010.1303074481673096No Hit
GGACTACCAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1010.1303074481673096No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA990.12772710265904605No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA970.1251467571507825No Hit
GGACTACTAGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGTGCCTCA970.1251467571507825No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA920.11869589338012358No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA920.11869589338012358No Hit
GGACTACTGGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA890.11482537511772826No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA880.11353520236359647No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA860.11095485685533293No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA830.10708433859293759No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA820.10579416583880583No Hit
GGACTACTGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC810.10450399308467404No Hit
GGACTACCGGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA800.10321382033054226No Hit
GGACTACAGGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA790.10192364757641047No Hit
GGACTACTCGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGCCTCA790.10192364757641047No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTT4700.0295.000038
CGTGAGC108.449829E-4295.0295
GGACTAC72050.0295.01
GTTCGCC255.275069E-10295.0295
ACTACCA5450.0295.03
TGTTAGC206.148002E-8295.0295
ACTAAGG108.449829E-4295.06
ACTCGGG7100.0295.06
CTACCGG3650.0295.04
TACTCGG7100.0295.05
AGTTAGT108.449829E-4295.0295
TACTAAG108.449829E-4295.05
TACACGG10600.0295.05
CGGGGTA1450.0294.999978
GTTACCC157.214392E-6294.99997295
AGTTAGG157.214392E-6294.99997295
GACTACT25300.0293.833982
CTACTAG12300.0293.80084
ACACGGG10650.0293.615056
GACTACC14700.0292.993232
Produced by FastQC (version 0.11.5)