FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491813

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491813
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64220
Sequences flagged as poor quality0
Sequence length301
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCCT157.2086295E-6295.01
TGCCCCT255.2568794E-10295.01
CACGGGC108.446455E-4295.06
CCGGCCT206.139817E-8295.01
ATAGCCT450.0295.01
CACGCCT157.2086295E-6295.01
GCGCCTA400.0295.02
CGTTCCT206.139817E-8295.01
ACGGGGC108.446455E-4295.08
GTGTGTC108.446455E-4295.0295
GCACCCT157.2086295E-6295.01
GTTACCT206.139817E-8295.01
CGGGGGC600.0295.09
CGGGGCA108.446455E-4295.09
AAGCCTA800.0295.02
GTAGCCT157.2086295E-6295.01
TACGCCT108.446455E-4295.01
AGGCCCC108.446455E-4295.0295
CGCTCCT255.2568794E-10295.01
TGCGCCT206.139817E-8295.01
Produced by FastQC (version 0.11.5)