FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491819

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491819
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73352
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC940.12814919838586541No Hit
TTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC890.121332751663213No Hit
CCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC860.11724288362962156No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC840.1145163049405606No Hit
TTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC830.11315301559603011No Hit
TATACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC820.11178972625149962No Hit
ATTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC790.10769985821790816No Hit
CCTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC790.10769985821790816No Hit
ATAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC780.10633656887337767No Hit
ATCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC780.10633656887337767No Hit
TCGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC780.10633656887337767No Hit
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC770.1049732795288472No Hit
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC770.1049732795288472No Hit
GTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC760.10360999018431671No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC760.10360999018431671No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC760.10360999018431671No Hit
CACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC750.10224670083978622No Hit
ACATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC750.10224670083978622No Hit
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC750.10224670083978622No Hit
TTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC750.10224670083978622No Hit
CTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC740.10088341149525576No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCCT350.0295.000031
CTTCCCT350.0295.000031
AAGACCT350.0295.000031
CGCGCCT350.0295.000031
TACGCCT350.0295.000031
CGCTCCT350.0295.000031
CCCCCCT350.0295.000031
GAGCCTA700.0295.000032
CATACCT350.0295.000031
TCTCCCT350.0295.000031
TTGCCTA700.0295.000032
TCCCCCT350.0295.000031
TCGCCTA1150.0295.02
TCTGCCT157.212817E-6295.01
TCGCCCT303.6379788E-12295.01
GCCCCCT206.1458195E-8295.01
AATCCTA900.0295.02
AGTCCTA1000.0295.02
TTGGCCT400.0295.01
GTCGCCT206.1458195E-8295.01
Produced by FastQC (version 0.11.5)