FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491823

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491823
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77735
Sequences flagged as poor quality0
Sequence length301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCCTA1100.0295.000032
ATCGCCT550.0295.000031
GTCGCCT108.4498705E-4295.01
TGCCCCT255.275069E-10295.01
CGCTACG108.4498705E-4295.03
CTAGGAG108.4498705E-4295.06
TGATCCT157.214463E-6294.999971
ACGCCCT303.6379788E-12294.999971
CGCGCCT303.6379788E-12294.999971
ACCTCGG157.214463E-6294.999974
GCCTAGG157.214463E-6294.999974
GACGCCT157.214463E-6294.999971
CGATCCT303.6379788E-12294.999971
TAGTCCT157.214463E-6294.999971
CCCTACG16550.0290.54384
CCTACGG73400.0288.56955
TACGGGA70650.0287.90167
CTACGGG71400.0287.76966
ACGGGAG70900.0286.470378
GACCTAC4750.0285.68423
Produced by FastQC (version 0.11.5)