FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491824

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491824
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77735
Sequences flagged as poor quality0
Sequence length301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACAAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG8401.0805943268797837No Hit
GGACTACAAGGGTATCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG7590.9763941596449477No Hit
GGACTACAAGGGTTTCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG7470.9609570978323793No Hit
GGACTACAAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG7280.9365150832958127No Hit
GGACTACTAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG6690.8606161960506851No Hit
GGACTACAAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT6660.856756930597543No Hit
GGACTACTAGGGTATCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG6550.8426062906026887No Hit
GGACTACAAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT6390.8220235415192642No Hit
GGACTACTAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT6190.7962951051649837No Hit
GGACTACTAGGGTTTCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG5840.7512703415449926No Hit
GGACTACTAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG5780.7435518106387085No Hit
GGACTACCAGGGTATCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG5360.6895220942947192No Hit
GGACTACCAGGGTTTCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG5290.6805171415707211No Hit
GGACTACCAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG5260.676657876117579No Hit
GGACTACTAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT5000.6432109088570142No Hit
GGACTACCAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG4830.6213417379558758No Hit
GGACTACCAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT4590.590467614330739No Hit
GGACTACACGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG4270.5493021161638901No Hit
GGACTACCAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT4270.5493021161638901No Hit
GGACTACACGGGTATCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG4160.5351514761690358No Hit
GGACTACACGGGTTTCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG3940.5068501961793273No Hit
GGACTACACGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT3900.5017045089084711No Hit
GGACTACACGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG3900.5017045089084711No Hit
GGACTACACGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT3480.4476747925644819No Hit
GGACTACTCGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG3450.4438155271113398No Hit
GGACTACTCGGGTATCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG3240.4168006689393452No Hit
GGACTACCCGGGTATCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG3020.3884993889496366No Hit
GGACTACTCGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2990.38464012349649446No Hit
GGACTACTCGGGTTTCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG2960.38078085804335243No Hit
GGACTACAAGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA2920.3756351707724963No Hit
GGACTACTCGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2910.37434874895478226No Hit
GGACTACAAGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA2850.3666302180484981No Hit
GGACTACAGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2730.3511931562359297No Hit
GGACTACCCGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2700.3473338907827877No Hit
GGACTACAGGGGTTTCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG2690.34604746896507366No Hit
GGACTACTCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2690.34604746896507366No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC2560.32932398533479124No Hit
GGACTACTAGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA2540.3267511416993632No Hit
GGACTACAGGGGTATCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG2420.3113140798867949No Hit
GGACTACAGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2410.3100276580690809No Hit
GGACTACCCGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2410.3100276580690809No Hit
GGACTACCCGGGTTTCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG2370.30488197079822477No Hit
GGACTACTAGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA2180.28043995626165824No Hit
GGACTACAGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2180.28043995626165824No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC2160.27786711262623015No Hit
GGACTACAAGGGTATCTAAGGTGATCAGGCCGCATCAGTCTTCAACCACT2130.27400784717308807No Hit
GGACTACAGGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2100.27014858171994593No Hit
GGACTACTGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2100.27014858171994593No Hit
GGACTACAAGGGTATCTAATGGTCCCCGGAGTTTCTCGGCACAGAGCTCA2100.27014858171994593No Hit
GGACTACCCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2090.26886215990223195No Hit
GGACTACTGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2060.26500289444908987No Hit
GGACTACCCGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2050.2637164726313758No Hit
GGACTACCAGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA2040.2624300508136618No Hit
GGACTACTGGGGTTTCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG2020.25985720717823374No Hit
GGACTACCGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2010.2585707853605197No Hit
GGACTACAAGGGTTTCTAAGGTGATCAGGCCGCATCAGTCTTCAACCACT1900.2444201453656654No Hit
GGACTACTGGGGTATCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG1880.24184730173023736No Hit
GGACTACCGGGGTTTCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG1870.2405608799125233No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1850.23798803627709525No Hit
GGACTACTGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1840.2367016144593812No Hit
GGACTACCGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1820.23412877082395317No Hit
GGACTACCAGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1800.23155592718852513No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1790.2302695053708111No Hit
GGACTACCAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1780.22898308355309704No Hit
GGACTACTAGGGTATCTAAGGTGATCAGGCCGCATCAGTCTTCAACCACT1740.22383739628224095No Hit
GGACTACCGGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1710.21997813082909887No Hit
GGACTACACGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1710.21997813082909887No Hit
GGACTACAAGGGTTTCTAATGGTCCCCGGAGTTTCTCGGCACAGAGCTCA1710.21997813082909887No Hit
GGACTACTGGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1710.21997813082909887No Hit
GGACTACCGGGGTATCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG1680.2161188653759568No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1660.21354602174052875No Hit
GGACTACTAGGGTTTCTAAGGTGATCAGGCCGCATCAGTCTTCAACCACT1620.2084003344696726No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1600.20582749083424456No Hit
GGACTACTAGGGTTTCTAATGGTCCCCGGAGTTTCTCGGCACAGAGCTCA1580.20325464719881647No Hit
GGACTACCAGGGTATCTAAGGTGATCAGGCCGCATCAGTCTTCAACCACT1560.20068180356338844No Hit
GGACTACCGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1550.1993953817456744No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1540.1981089599279604No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1540.1981089599279604No Hit
GGACTACAAGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1500.19296327265710425No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1490.19167685083939023No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1490.19167685083939023No Hit
GGACTACAAGGGTATCTAATTTTCCTCTCACCTCCCTACTGCTCCTTCTC1460.18781758538624815No Hit
GGACTACACGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1450.18653116356853414No Hit
GGACTACCAGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1450.18653116356853414No Hit
GGACTACACGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1420.18267189811539206No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1410.181385476297678No Hit
GGACTACTAGGGTATCTAATGGTCCCCGGAGTTTCTCGGCACAGAGCTCA1380.1775262108445359No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1380.1775262108445359No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1350.17366694539139385No Hit
GGACTACAAGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1340.1723805235736798No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1340.1723805235736798No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1310.16852125812053773No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1290.16594841448510966No Hit
GGACTACAAGGGTATCTAATGTGATCAGGCCGCATCAGTCTTCAACCACT1290.16594841448510966No Hit
GGACTACAAGGGTATCTAACATACGTGTACCAGGTTGGCATGCCTCCAGC1290.16594841448510966No Hit
GGACTACTCGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1280.16466199266739562No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1280.16466199266739562No Hit
GGACTACACGGGTTTCTAAGGTGATCAGGCCGCATCAGTCTTCAACCACT1280.16466199266739562No Hit
GGACTACTAGGGTTTCTAATTTTCCTCTCACCTCCCTACTGCTCCTTCTC1250.16080272721425354No Hit
GGACTACTAGGGTATCTAATTTTCCTCTCACCTCCCTACTGCTCCTTCTC1250.16080272721425354No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1250.16080272721425354No Hit
GGACTACTAGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1250.16080272721425354No Hit
GGACTACCAGGGTATCTAATGGTCCCCGGAGTTTCTCGGCACAGAGCTCA1250.16080272721425354No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1230.1582298835788255No Hit
GGACTACAAGGGTTTCTAATTTTCCTCTCACCTCCCTACTGCTCCTTCTC1230.1582298835788255No Hit
GGACTACTCGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1210.15565703994339744No Hit
GGACTACAAGGGTTTCTAATGTGATCAGGCCGCATCAGTCTTCAACCACT1200.15437061812568342No Hit
GGACTACACGGGTATCTAAGGTGATCAGGCCGCATCAGTCTTCAACCACT1190.15308419630796938No Hit
GGACTACCAGGGTTTCTAATGGTCCCCGGAGTTTCTCGGCACAGAGCTCA1180.15179777449025536No Hit
GGACTACAAGGGTATCTAATGGCTGACAGATTATCCTGTGTGCTTGGAGG1180.15179777449025536No Hit
GGACTACCAGGGTTTCTAAGGTGATCAGGCCGCATCAGTCTTCAACCACT1150.14793850903711325No Hit
GGACTACTCGGGTTTCTAAGGTGATCAGGCCGCATCAGTCTTCAACCACT1150.14793850903711325No Hit
GGACTACCAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1140.14665208721939924No Hit
GGACTACCCGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1120.14407924358397117No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1100.14150639994854314No Hit
GGACTACTAGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1090.14021997813082912No Hit
GGACTACAAGGGTTTCTAATGGCTGACAGATTATCCTGTGTGCTTGGAGG1090.14021997813082912No Hit
GGACTACCAGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1080.13893355631311508No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1080.13893355631311508No Hit
GGACTACTAGGGTATCTAATGGCTGACAGATTATCCTGTGTGCTTGGAGG1070.13764713449540106No Hit
GGACTACAAGGGTATCTAATACCGGATGTGCTCTGACTCGCCCACTTCCG1070.13764713449540106No Hit
GGACTACAAGGGTTTCTAACATACGTGTACCAGGTTGGCATGCCTCCAGC1050.13507429085997297No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1050.13507429085997297No Hit
GGACTACAGGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1040.13378786904225895No Hit
GGACTACCAGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1040.13378786904225895No Hit
GGACTACACGGGTATCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1030.13250144722454493No Hit
GGACTACACGGGTATCTAATGGTCCCCGGAGTTTCTCGGCACAGAGCTCA1030.13250144722454493No Hit
GGACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1030.13250144722454493No Hit
GGACTACTAGGGTTTCTAATGTGATCAGGCCGCATCAGTCTTCAACCACT1010.12992860358911687No Hit
GGACTACTGGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1000.12864218177140285No Hit
GGACTACACGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC990.1273557599536888No Hit
GGACTACCAGGGTATCTAATGTGATCAGGCCGCATCAGTCTTCAACCACT970.12478291631826076No Hit
GGACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC960.12349649450054674No Hit
GGACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC950.1222100726828327No Hit
GGACTACAGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC940.12092365086511868No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA940.12092365086511868No Hit
GGACTACCAGGGTATCTAATTTTCCTCTCACCTCCCTACTGCTCCTTCTC930.11963722904740465No Hit
GGACTACCCGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA930.11963722904740465No Hit
GGACTACTAGGGTTTCTAATGGCTGACAGATTATCCTGTGTGCTTGGAGG920.1183508072296906No Hit
GGACTACTAGGGTATCTAATGTGATCAGGCCGCATCAGTCTTCAACCACT920.1183508072296906No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC910.11706438541197658No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC910.11706438541197658No Hit
GGACTACTAGGGTATCTAACATACGTGTACCAGGTTGGCATGCCTCCAGC900.11577796359426257No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC900.11577796359426257No Hit
GGACTACACGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC890.11449154177654852No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA890.11449154177654852No Hit
GGACTACACGGGTATCTAATTTTCCTCTCACCTCCCTACTGCTCCTTCTC890.11449154177654852No Hit
GGACTACCAGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC890.11449154177654852No Hit
GGACTACAAGGGTATCTAACTGAAGGGGCGATTTCCCTTCACATGTCCAC880.1132051199588345No Hit
GGACTACTCGGGTATCTAAGGTGATCAGGCCGCATCAGTCTTCAACCACT880.1132051199588345No Hit
GGACTACACGGGTTTCTAATGGTCCCCGGAGTTTCTCGGCACAGAGCTCA880.1132051199588345No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA870.11191869814112047No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC850.10934585450569241No Hit
GGACTACCAGGGTATCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC850.10934585450569241No Hit
GGACTACCAGGGTATCTAATGGCTGACAGATTATCCTGTGTGCTTGGAGG850.10934585450569241No Hit
GGACTACCAGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC840.1080594326879784No Hit
GGACTACTCGGGTATCTAATGGTCCCCGGAGTTTCTCGGCACAGAGCTCA830.10677301087026438No Hit
GGACTACCCGGGTATCTAAGGTGATCAGGCCGCATCAGTCTTCAACCACT820.10548658905255033No Hit
GGACTACCAGGGTTTCTAATTTTCCTCTCACCTCCCTACTGCTCCTTCTC810.1042001672348363No Hit
GGACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC800.10291374541712228No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA800.10291374541712228No Hit
GGACTACTCGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC780.10034090178169422No Hit
GGACTACAAGGGTTTCTAACTGAAGGGGCGATTTCCCTTCACATGTCCAC780.10034090178169422No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC780.10034090178169422No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGTT3200.0295.08
CATTCGG108.4498705E-4295.0295
ACGGGTT4900.0295.08
CCGGGTT2400.0294.999978
GGACTAC74200.0294.602421
ACACGGG9650.0293.47156
CGGGTTT10600.0292.216989
TACACGG9750.0291.974375
ACGGGTA4700.0291.86178
AGGGTAT20500.0291.402449
TACAAGG15750.0291.253975
CAAGGGT15750.0290.317477
ACAAGGG15750.0290.317476
GGGGTAT6200.0290.241949
CGGGTAT12300.0290.203259
CAGGGGT5850.0289.957247
GGGGTTT7250.0288.896559
CACGGGT9800.0287.47457
TACTAGG13300.0287.236855
GACTACC18900.0287.195772
Produced by FastQC (version 0.11.5)