FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491829

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491829
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82488
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT960.11638056444573756No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCCT550.0295.000031
CGTACCT550.0295.000031
CGAACCT350.0295.01
GCGACCT255.275069E-10295.01
CGACCTC157.216062E-6295.0295
CGAGCCT157.216062E-6295.01
CCTACGG78550.0290.117775
TACGGGA76150.0289.963877
CTACGGG77250.0289.653726
GCCTACG15300.0289.21574
TCCTACG23400.0288.69664
CCCTACG18250.0288.534244
ACCTACG22350.0287.740484
ACGGGAG76700.0287.58
GTCCTAC5200.0286.49043
CGTCCTA1400.0284.46432
CGGGAGG76050.0284.138739
GCGCCTA1000.0280.252
ACCCTAC4800.0279.635443
CGCCTAC3950.0276.329133
Produced by FastQC (version 0.11.5)