FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491833

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491833
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73087
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1380.18881606852107763No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1270.1737655123346149No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1230.16829258281226483No Hit
TAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1200.16418788567050227No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1190.16281965328991474No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1190.16281965328991474No Hit
TCGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1180.16145142090932724No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1140.15597849138697717No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1130.15461025900638967No Hit
CAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1120.15324202662580214No Hit
TTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1110.1518737942452146No Hit
CTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1110.1518737942452146No Hit
CCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1100.15050556186462707No Hit
TAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1100.15050556186462707No Hit
TACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1100.15050556186462707No Hit
TCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1090.14913732948403957No Hit
AAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1090.14913732948403957No Hit
CTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1090.14913732948403957No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1080.14776909710345204No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1080.14776909710345204No Hit
CTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1080.14776909710345204No Hit
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1080.14776909710345204No Hit
CAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1070.14640086472286454No Hit
TCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1070.14640086472286454No Hit
CCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1050.1436643999616895No Hit
ATACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1050.1436643999616895No Hit
TTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1030.14092793520051444No Hit
ACGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1020.13955970281992694No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1020.13955970281992694No Hit
ATTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1020.13955970281992694No Hit
TATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1020.13955970281992694No Hit
CTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1000.1368232380587519No Hit
ATCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1000.1368232380587519No Hit
AACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1000.1368232380587519No Hit
ATGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1000.1368232380587519No Hit
AAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC990.13545500567816438No Hit
TCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC990.13545500567816438No Hit
CACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC980.13408677329757684No Hit
CCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC970.13271854091698934No Hit
GTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC970.13271854091698934No Hit
TTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC950.1299820761558143No Hit
TTAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC950.1299820761558143No Hit
TCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC950.1299820761558143No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC940.12861384377522678No Hit
CTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC940.12861384377522678No Hit
GGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC940.12861384377522678No Hit
GTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC930.12724561139463927No Hit
TTCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC920.12587737901405174No Hit
TGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC920.12587737901405174No Hit
ACTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC910.12450914663346424No Hit
AACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC910.12450914663346424No Hit
TCGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC910.12450914663346424No Hit
CTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC910.12450914663346424No Hit
TTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC910.12450914663346424No Hit
TTGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC910.12450914663346424No Hit
AAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC910.12450914663346424No Hit
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC910.12450914663346424No Hit
ATCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC910.12450914663346424No Hit
GGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC910.12450914663346424No Hit
TGCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC910.12450914663346424No Hit
CGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC900.12314091425287671No Hit
GTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC900.12314091425287671No Hit
TACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC890.12177268187228919No Hit
GGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC890.12177268187228919No Hit
AATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC890.12177268187228919No Hit
ACCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC890.12177268187228919No Hit
CAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC890.12177268187228919No Hit
CCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC880.12040444949170168No Hit
TGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC880.12040444949170168No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC880.12040444949170168No Hit
TCCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC880.12040444949170168No Hit
GTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC880.12040444949170168No Hit
GTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC880.12040444949170168No Hit
TCTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC870.11903621711111415No Hit
ACAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC870.11903621711111415No Hit
CCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC860.11766798473052663No Hit
GATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC860.11766798473052663No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC860.11766798473052663No Hit
CATCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC860.11766798473052663No Hit
ATTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC860.11766798473052663No Hit
TTCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC860.11766798473052663No Hit
GCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC860.11766798473052663No Hit
AGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC860.11766798473052663No Hit
CTTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC860.11766798473052663No Hit
CGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC860.11766798473052663No Hit
GCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC850.11629975234993913No Hit
ATGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC850.11629975234993913No Hit
TGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC850.11629975234993913No Hit
CTCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC850.11629975234993913No Hit
TAGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC850.11629975234993913No Hit
AGTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC850.11629975234993913No Hit
CATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC850.11629975234993913No Hit
CGCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC850.11629975234993913No Hit
TATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC840.1149315199693516No Hit
AAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC840.1149315199693516No Hit
ACACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC840.1149315199693516No Hit
ACAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC840.1149315199693516No Hit
ACCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC840.1149315199693516No Hit
AGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC830.11356328758876408No Hit
TGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC830.11356328758876408No Hit
CACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC830.11356328758876408No Hit
GTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC820.11219505520817655No Hit
CAGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC820.11219505520817655No Hit
GTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC820.11219505520817655No Hit
GAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC820.11219505520817655No Hit
AGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC820.11219505520817655No Hit
TAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC820.11219505520817655No Hit
GAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC820.11219505520817655No Hit
CTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC810.11082682282758903No Hit
CTTGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC810.11082682282758903No Hit
GCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC810.11082682282758903No Hit
CGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC810.11082682282758903No Hit
TTTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC810.11082682282758903No Hit
TCCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC800.10945859044700153No Hit
CATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC800.10945859044700153No Hit
ATCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC800.10945859044700153No Hit
CAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC800.10945859044700153No Hit
CCAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC800.10945859044700153No Hit
CCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC800.10945859044700153No Hit
GAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC800.10945859044700153No Hit
AGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC790.10809035806641401No Hit
AACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC790.10809035806641401No Hit
GACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC790.10809035806641401No Hit
ACGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC790.10809035806641401No Hit
CGTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC790.10809035806641401No Hit
ATGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC790.10809035806641401No Hit
GAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC790.10809035806641401No Hit
AAGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC790.10809035806641401No Hit
CGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC780.10672212568582648No Hit
AGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC780.10672212568582648No Hit
TCAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC780.10672212568582648No Hit
CGACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC770.10535389330523896No Hit
ACGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC770.10535389330523896No Hit
CCCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC770.10535389330523896No Hit
GGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC770.10535389330523896No Hit
TGACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC770.10535389330523896No Hit
TTTGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC770.10535389330523896No Hit
TAAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC760.10398566092465143No Hit
GCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC760.10398566092465143No Hit
TGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC760.10398566092465143No Hit
CAAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC750.10261742854406392No Hit
ATTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC750.10261742854406392No Hit
AGACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC750.10261742854406392No Hit
GACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC750.10261742854406392No Hit
GTCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC750.10261742854406392No Hit
GTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC750.10261742854406392No Hit
GCGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC750.10261742854406392No Hit
CTAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC740.10124919616347641No Hit
CGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC740.10124919616347641No Hit
GGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC740.10124919616347641No Hit
ATCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC740.10124919616347641No Hit
TAGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC740.10124919616347641No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGG700.0295.00003295
TGCCCCT350.0295.000031
AGATCCT350.0295.000031
GTTTCCT350.0295.000031
ATTACCT350.0295.000031
GCGCCTA700.0295.000032
GACCCCT350.0295.000031
TCGTCCT350.0295.000031
TACTCCT350.0295.000031
AGCTCCT350.0295.000031
CGCACCT350.0295.000031
GGACCCT350.0295.000031
AGGGCCT350.0295.000031
CGCCCTA700.0295.000032
CTAGCCT350.0295.000031
AGGTCCT350.0295.000031
AGTCCTA850.0295.02
ATCTCCT500.0295.01
GTCGCCT255.2568794E-10295.01
CGAACCT255.2568794E-10295.01
Produced by FastQC (version 0.11.5)