FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005491834

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491834
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73087
Sequences flagged as poor quality0
Sequence length301
%GC50

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA28823.94324572085323No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA22383.0621040677548677No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA19592.6803672335709496No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA15272.0892908451571413No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA14481.9812004870907274No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA13971.9114206356807641No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA13311.821117298561988No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA11501.573467237675647No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA10481.4339075348557198No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA10191.3942287958186819No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA9641.3189760148863683No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA9221.2615102549016926No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA8891.2163585863423043No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA8681.1876257063499664No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA8571.1725751501635038No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA8181.1192140873205905No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA8131.1123729254176529No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA7461.0207013559182891No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA6970.9536579692695007No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA6940.9495532721277382No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA6930.9481850397471507No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6370.8715640264342496No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA6310.8633546321507245No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA5950.8140982664495737No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA5850.8004159426436986No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5770.7894700835989985No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5720.7826289216960609No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA5190.7101126055249224No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5170.7073761407637473No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA4820.6594880074431841No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA4580.6266504303090837No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA4550.6225457331673212No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA4510.6170728036449711No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4490.614336338883796No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA4320.5910763884138082No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4160.5691846703244079No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4050.5541341141379451No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA4040.5527658817573577No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA4010.5486611846155951No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3840.5254012341456074No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA3840.5254012341456074No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA3730.5103506779591446No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA3730.5103506779591446No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA3620.49530012177268185No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA3610.4939318893920943No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA3330.45562138273564384No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3300.4515166855938813No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA3300.4515166855938813No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3280.4487802208327062No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA3230.44193905892976865No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3150.43099319988506846No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA3140.4296249675044809No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA3080.42141557322095585No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3060.41867910845978085No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3050.4173108760791933No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA3040.41594264369860573No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2970.4063650170344931No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA2890.395419157989793No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2880.39405092560920546No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA2870.39268269322861793No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2840.3885779960868554No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2750.3762639046615677No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2650.36258158085569253No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA2630.3598451160945175No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA2580.3530039541915799No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2570.35163572181099234No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA2550.34889925704981734No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2530.34616279228864233No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2530.34616279228864233No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2520.3447945599080548No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCA2450.33521693324394214No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2450.33521693324394214No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2440.3338487008633546No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA2410.32974400372159207No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA2390.32700753896041707No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2370.324271074199242No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2320.3174299122963044No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2280.31195698277395434No Hit
GGACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA2230.30511582087101674No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2220.3037475884904292No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2210.3023793561098417No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2210.3023793561098417No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA2170.2969064265874916No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2120.290065264684554No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2040.2791194056398539No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2010.2750147084980913No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1850.253122990408691No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA1820.24901829326692848No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA1820.24901829326692848No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA1790.2449135961251659No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1740.2380724342222283No Hit
GGACTACAAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1730.2367042018416408No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1710.23396773708046575No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1700.23259950469987825No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCA1700.23259950469987825No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1670.22849480755811566No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1660.22712657517752816No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1650.22575834279694065No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1610.22028541327459056No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA1580.21618071613282802No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1570.21481248375224046No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1560.21344425137165296No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA1550.21207601899106543No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA1500.20523485708812783No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1490.20386662470754033No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCA1460.1997619275657778No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1460.1997619275657778No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCA1430.19565723042401523No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1430.19565723042401523No Hit
GGACTACAAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA1420.1942889980434277No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1410.19292076566284017No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1380.18881606852107763No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1380.18881606852107763No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1360.18607960375990257No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA1350.18471137137931506No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1330.18197490661814003No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCA1330.18197490661814003No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA1310.17923844185696497No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1290.17650197709578996No Hit
GGACTACTAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1260.1723972799540274No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA1250.17102904757343987No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA1220.1669243504316773No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1190.16281965328991474No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCA1150.15734672376756467No Hit
GGACTACAAGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGTGCATCA1110.1518737942452146No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA1080.14776909710345204No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1070.14640086472286454No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA1070.14640086472286454No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA1060.145032632342277No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA1050.1436643999616895No Hit
GGACTACAAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1050.1436643999616895No Hit
GGACTACTAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA1040.14229616758110197No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1020.13955970281992694No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1020.13955970281992694No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCA1000.1368232380587519No Hit
GGACTACCAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG970.13271854091698934No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA930.12724561139463927No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA910.12450914663346424No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA900.12314091425287671No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA890.12177268187228919No Hit
GGACTACTAGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA860.11766798473052663No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCA850.11629975234993913No Hit
GGACTACTAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG850.11629975234993913No Hit
GGACTACAGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG850.11629975234993913No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA840.1149315199693516No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA830.11356328758876408No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA810.11082682282758903No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCA810.11082682282758903No Hit
GGACTACACGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA810.11082682282758903No Hit
GGACTACACGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG810.11082682282758903No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCA810.11082682282758903No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA800.10945859044700153No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGCTACCCACGCTCTCGTGCATCA780.10672212568582648No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA780.10672212568582648No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA760.10398566092465143No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCA750.10261742854406392No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCA750.10261742854406392No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)