FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491835

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491835
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66746
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC870.13034488958139814No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC840.12585023821652233No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC810.12135558685164655No Hit
TTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC790.11835915260839601No Hit
CTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC790.11835915260839601No Hit
TAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC780.11686093548677073No Hit
TTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC760.11386450124352021No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC740.11086806700026969No Hit
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC740.11086806700026969No Hit
CACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC730.10936984987864441No Hit
TATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC730.10936984987864441No Hit
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC720.10787163275701914No Hit
ATGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC710.10637341563539389No Hit
GTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC710.10637341563539389No Hit
TCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC710.10637341563539389No Hit
ATTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC710.10637341563539389No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC710.10637341563539389No Hit
ATCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC700.10487519851376861No Hit
TTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC700.10487519851376861No Hit
TACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC700.10487519851376861No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC700.10487519851376861No Hit
CCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC700.10487519851376861No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC690.10337698139214334No Hit
CAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC690.10337698139214334No Hit
TCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC690.10337698139214334No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC680.10187876427051809No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC680.10187876427051809No Hit
CTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC680.10187876427051809No Hit
TGTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC670.10038054714889282No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCTAC4450.0294.994783
TCTGCCT450.0294.994751
GCCCCTA450.0294.994752
GTCGCCT255.2568794E-10294.994751
AGATCCT157.2104012E-6294.994751
ACGCCTA900.0294.994752
CTTCCTA1100.0294.994752
CTTCCCT255.2568794E-10294.994751
CGGCCTA500.0294.994752
AGCCCTA900.0294.994752
GAGTCCT157.2104012E-6294.994751
CTCCCTA1100.0294.994752
GGTCCTA1000.0294.994752
GGTCCCT108.447643E-4294.994751
TCCCTAC3200.0294.994753
AAGACCT206.1421815E-8294.994751
CACGCCT206.1421815E-8294.994751
CGCGCCT206.1421815E-8294.994751
GGATCCT206.1421815E-8294.994751
TGAACCT350.0294.994751
Produced by FastQC (version 0.11.5)