FastQCFastQC Report
Wed 8 May 2024
EGAF00005491837

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491837
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72569
Total Bases21.8 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT840.11575190508343783No Hit
TTTTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT800.11023990960327412No Hit
CTATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT790.10886191073323319No Hit
CCAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT780.10748391186319228No Hit
TTTACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT770.10610591299315135No Hit
TTCTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT770.10610591299315135No Hit
ATCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT740.10197191638302856No Hit
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT730.10059391751298764No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)