FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491841

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491841
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64821
Sequences flagged as poor quality0
Sequence length301
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC780.12033137409172953No Hit
TTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC750.11570324431897071No Hit
CTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC750.11570324431897071No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC740.11416053439471775No Hit
CTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC730.11261782447046483No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC720.11107511454621188No Hit
TCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC710.10953240462195893No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC700.10798969469770599No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC700.10798969469770599No Hit
CAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC690.10644698477345306No Hit
ATTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC690.10644698477345306No Hit
AACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC690.10644698477345306No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC670.10336156492494715No Hit
ATTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC660.10181885500069422No Hit
GCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC660.10181885500069422No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC660.10181885500069422No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC660.10181885500069422No Hit
TTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC660.10181885500069422No Hit
TCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC650.10027614507644128No Hit
CTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC650.10027614507644128No Hit
TTTGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC650.10027614507644128No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCTA700.0295.000032
CGTTCCT350.0295.000031
AGAACCT350.0295.000031
CGTCCTA1250.0295.000032
CCGCCTA950.0295.000032
TCGACCT350.0295.000031
GCCCCTA850.0295.02
GCCCCCT108.446637E-4295.01
AATCCCT255.2568794E-10295.01
GCGACCT108.446637E-4295.01
TGCCACG108.446637E-4295.03
TACGGGC108.446637E-4295.06
TAGCCCT108.446637E-4295.01
ACGGGAT108.446637E-4295.07
ACGGGAC108.446637E-4295.08
CACCTAG108.446637E-4295.03
GTTACCT108.446637E-4295.01
GTAGCCT255.2568794E-10295.01
TCTACCT500.0295.01
ATCCCTA800.0295.02
Produced by FastQC (version 0.11.5)