FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005491844

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491844
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61170
Sequences flagged as poor quality0
Sequence length301
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACTCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG10301.6838319437632827No Hit
GGACTACCCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG10231.672388425698872No Hit
GGACTACACGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG8091.322543730586889No Hit
GGACTACTGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG7131.1656040542749715No Hit
GGACTACTAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG6351.0380905672715384No Hit
GGACTACTCGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC6181.0102991662579697No Hit
GGACTACCCGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC5890.9628903057054111No Hit
GGACTACAAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG5570.9105770802681052No Hit
GGACTACTCGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG5450.8909596207291155No Hit
GGACTACCGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG5280.8631682197155469No Hit
GGACTACACGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC4960.8108549942782409No Hit
GGACTACAGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG4750.776524440085009No Hit
GGACTACCCGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT4650.7601765571358509No Hit
GGACTACTCGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT4330.707863331698545No Hit
GGACTACTGGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC4270.6980546019290502No Hit
GGACTACTAGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC3860.631028281837502No Hit
GGACTACACGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT3840.6277587052476705No Hit
GGACTACTCGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC3610.5901585744646068No Hit
GGACTACAAGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC3230.5280366192578061No Hit
GGACTACCCGGGTTTCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG3180.519862677783227No Hit
GGACTACCGGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC3150.5149583128984796No Hit
GGACTACTGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2980.4871669118849109No Hit
GGACTACTAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2940.48062775870524765No Hit
GGACTACTCGGGTTTCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG2860.4675494523459212No Hit
GGACTACAGGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC2830.4626450874611738No Hit
GGACTACTCGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2610.426679744973026No Hit
GGACTACCCGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2470.40379270884420465No Hit
GGACTACCGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2340.38254046101029915No Hit
GGACTACTGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2220.36292300147130946No Hit
GGACTACAAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2200.35965342488147783No Hit
GGACTACTAGGGTTTCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG2110.3449403302272356No Hit
GGACTACTGGGGTTTCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG2050.3351316004577407No Hit
GGACTACAGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1960.32041850580349845No Hit
GGACTACACGGGTTTCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG1920.31387935262383526No Hit
GGACTACACGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1750.28608795161026646No Hit
GGACTACCCGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1720.281183586725519No Hit
GGACTACTCGGGTATCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG1710.2795487984306032No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1630.26647049207127677No Hit
GGACTACCCGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1610.26320091548144514No Hit
GGACTACCCGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1570.25666176230178195No Hit
GGACTACCGGGGTTTCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG1520.2484878208272029No Hit
GGACTACAAGGGTTTCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG1480.24194866764753964No Hit
GGACTACCCGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1450.23704430276279223No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1420.2321399378780448No Hit
GGACTACTGGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1420.2321399378780448No Hit
GGACTACCCGGGTTTCTAATGGCTGACAGATTATCCTGTGTGCTTGGAGG1400.22887036128821317No Hit
GGACTACTCGGGTTTCTAATGGCTGACAGATTATCCTGTGTGCTTGGAGG1340.21906163151871835No Hit
GGACTACAGGGGTTTCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG1330.21742684322380254No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1290.2108876900441393No Hit
GGACTACACGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1280.20925290174922348No Hit
GGACTACTCGGGTTTCTAAAACAATTTGTTGAGGGTAGAACAACTCAGTT1220.19944417197972863No Hit
GGACTACAAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1200.196174595389897No Hit
GGACTACTCGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1160.18963544221023376No Hit
GGACTACTGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1160.18963544221023376No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1160.18963544221023376No Hit
GGACTACTAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1150.18800065391531795No Hit
GGACTACCCGGGTTTCTAACTGAAGGGGCGATTTCCCTTCACATGTCCAC1120.18309628903057054No Hit
GGACTACTCGGGTTTCTACATTGGTAAGACATACGCCTGGGGTTCCTGGC1100.17982671244073892No Hit
GGACTACTCGGGTTTCTAACTGAAGGGGCGATTTCCCTTCACATGTCCAC1050.17165277096615988No Hit
GGACTACACGGGTTTCTACATTGGTAAGACATACGCCTGGGGTTCCTGGC1040.17001798267124407No Hit
GGACTACACGGGTTTCTAATGGCTGACAGATTATCCTGTGTGCTTGGAGG990.16184404119666504No Hit
GGACTACCCGGGTTTCTACATTGGTAAGACATACGCCTGGGGTTCCTGGC980.16020925290174923No Hit
GGACTACTCGGGTTTCTAAGTCTGGTCCTTCAACCAAGGGACTGACAACT970.15857446460683342No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC970.15857446460683342No Hit
GGACTACTGGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT970.15857446460683342No Hit
GGACTACCCGGGTTTCTAAAACAATTTGTTGAGGGTAGAACAACTCAGTT950.15530488801700182No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC950.15530488801700182No Hit
GGACTACCCGGGTTTCTAAGTCTGGTCCTTCAACCAAGGGACTGACAACT950.15530488801700182No Hit
GGACTACCCGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC930.1520353114271702No Hit
GGACTACAGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG930.1520353114271702No Hit
GGACTACACGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA910.14876573483733857No Hit
GGACTACTCGGGTTTCTAAAAGGCTAGATAGTAACTGTGGAATTTTGCAA900.14713094654242276No Hit
GGACTACTGGGGTTTCTACATTGGTAAGACATACGCCTGGGGTTCCTGGC890.14549615824750695No Hit
GGACTACTAGGGTTTCTAATGGCTGACAGATTATCCTGTGTGCTTGGAGG880.14386136995259113No Hit
GGACTACACGGGTTTCTAAAACAATTTGTTGAGGGTAGAACAACTCAGTT870.14222658165767532No Hit
GGACTACCCGGGTTTCTAAGACTCTTGTCAACGTATAAGCCTTCCTGTTG860.1405917933627595No Hit
GGACTACCCGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC860.1405917933627595No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC850.1389570050678437No Hit
GGACTACACGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT850.1389570050678437No Hit
GGACTACAAGGGTTTCTAATGGCTGACAGATTATCCTGTGTGCTTGGAGG830.1356874284780121No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC820.1340526401830963No Hit
GGACTACCGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC820.1340526401830963No Hit
GGACTACTCGGGTTTCTAAGACTCTTGTCAACGTATAAGCCTTCCTGTTG820.1340526401830963No Hit
GGACTACAGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC820.1340526401830963No Hit
GGACTACCCGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT820.1340526401830963No Hit
GGACTACTGGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC820.1340526401830963No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC800.13078306359326466No Hit
GGACTACTCGGGTTTCTAATACCGGATGTGCTCTGACTCGCCCACTTCCG800.13078306359326466No Hit
GGACTACTGGGGTTTCTAATGGCTGACAGATTATCCTGTGTGCTTGGAGG790.12914827529834885No Hit
GGACTACCGGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC780.12751348700343307No Hit
GGACTACCCGGGTTTCTAAAAGGCTAGATAGTAACTGTGGAATTTTGCAA770.12587869870851726No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC770.12587869870851726No Hit
GGACTACTAGGGTTTCTAAGACTCTTGTCAACGTATAAGCCTTCCTGTTG770.12587869870851726No Hit
GGACTACTAGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA760.12424391041360144No Hit
GGACTACACGGGTTTCTAAAAGGCTAGATAGTAACTGTGGAATTTTGCAA740.12097433382376982No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC740.12097433382376982No Hit
GGACTACTGGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA730.11933954552885401No Hit
GGACTACACGGGTTTCTAACTGAAGGGGCGATTTCCCTTCACATGTCCAC730.11933954552885401No Hit
GGACTACTCGGGTATCTAACTGAAGGGGCGATTTCCCTTCACATGTCCAC730.11933954552885401No Hit
GGACTACTCGGGTATCTAATGGCTGACAGATTATCCTGTGTGCTTGGAGG730.11933954552885401No Hit
GGACTACACGGGTTTCTAAGACTCTTGTCAACGTATAAGCCTTCCTGTTG720.11770475723393821No Hit
GGACTACACGGGTTTCTAAGTCTGGTCCTTCAACCAAGGGACTGACAACT700.11443518064410658No Hit
GGACTACTGGGGTTTCTAAAACAATTTGTTGAGGGTAGAACAACTCAGTT700.11443518064410658No Hit
GGACTACCCGGGTTTCTAACATACGTGTACCAGGTTGGCATGCCTCCAGC690.11280039234919077No Hit
GGACTACAAGGGTTTCTACATTGGTAAGACATACGCCTGGGGTTCCTGGC690.11280039234919077No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC680.11116560405427496No Hit
GGACTACTAGGGTTTCTAAAAGGCTAGATAGTAACTGTGGAATTTTGCAA680.11116560405427496No Hit
GGACTACTGGGGTATCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG670.10953081575935918No Hit
GGACTACTAGGGTTTCTACATTGGTAAGACATACGCCTGGGGTTCCTGGC670.10953081575935918No Hit
GGACTACAAGGGTTTCTAAAACAATTTGTTGAGGGTAGAACAACTCAGTT670.10953081575935918No Hit
GGACTACAGGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC660.10789602746444336No Hit
GGACTACTAGGGTTTCTAAAACAATTTGTTGAGGGTAGAACAACTCAGTT660.10789602746444336No Hit
GGACTACAAGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA660.10789602746444336No Hit
GGACTACCCGGGTTTCTAATACCGGATGTGCTCTGACTCGCCCACTTCCG650.10626123916952755No Hit
GGACTACAAGGGTTTCTAAAAGGCTAGATAGTAACTGTGGAATTTTGCAA640.10462645087461174No Hit
GGACTACTGGGGTTTCTAAGACTCTTGTCAACGTATAAGCCTTCCTGTTG640.10462645087461174No Hit
GGACTACTAGGGTTTCTAACTGAAGGGGCGATTTCCCTTCACATGTCCAC640.10462645087461174No Hit
GGACTACTCGGGTATCTAAAAGGCTAGATAGTAACTGTGGAATTTTGCAA630.10299166257969593No Hit
GGACTACTGGGGTTTCTAAAAGGCTAGATAGTAACTGTGGAATTTTGCAA630.10299166257969593No Hit
GGACTACAAGGGTTTCTAAGTCTGGTCCTTCAACCAAGGGACTGACAACT620.10135687428478012No Hit
GGACTACAAGGGTTTCTAAGACTCTTGTCAACGTATAAGCCTTCCTGTTG620.10135687428478012No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC620.10135687428478012No Hit
GGACTACTGGGGTTTCTAACTGAAGGGGCGATTTCCCTTCACATGTCCAC620.10135687428478012No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)