FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491847

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491847
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58322
Sequences flagged as poor quality0
Sequence length301
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC870.14917183910016804No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC840.14402798257947258No Hit
ATCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC830.14231336373924078No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC790.1354548883783135No Hit
CCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC790.1354548883783135No Hit
CCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC780.13374026953808169No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC780.13374026953808169No Hit
AACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC780.13374026953808169No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC760.13031103185761805No Hit
TTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC760.13031103185761805No Hit
CTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC750.12859641301738622No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC750.12859641301738622No Hit
ATACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC750.12859641301738622No Hit
AAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC740.12688179417715442No Hit
TAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC740.12688179417715442No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC730.1251671753369226No Hit
GTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC730.1251671753369226No Hit
AACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC730.1251671753369226No Hit
CATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC720.12345255649669079No Hit
CTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC720.12345255649669079No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC720.12345255649669079No Hit
ATTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC720.12345255649669079No Hit
CGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC720.12345255649669079No Hit
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC720.12345255649669079No Hit
GGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC710.12173793765645896No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC710.12173793765645896No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC700.12002331881622716No Hit
CTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC700.12002331881622716No Hit
CTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC700.12002331881622716No Hit
TACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC690.11830869997599533No Hit
GTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC680.11659408113576353No Hit
CATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC680.11659408113576353No Hit
CACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC680.11659408113576353No Hit
ATTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC680.11659408113576353No Hit
CCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC680.11659408113576353No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC680.11659408113576353No Hit
ACCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC670.1148794622955317No Hit
AATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC660.1131648434552999No Hit
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC660.1131648434552999No Hit
CGTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC660.1131648434552999No Hit
CAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC660.1131648434552999No Hit
TAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC650.11145022461506807No Hit
ACAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC650.11145022461506807No Hit
CAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC650.11145022461506807No Hit
CTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC640.10973560577483626No Hit
TCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC640.10973560577483626No Hit
GAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC630.10802098693460443No Hit
ACGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC630.10802098693460443No Hit
GCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC630.10802098693460443No Hit
GTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC630.10802098693460443No Hit
CTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC630.10802098693460443No Hit
GAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC630.10802098693460443No Hit
CCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC630.10802098693460443No Hit
ATCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC630.10802098693460443No Hit
TACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC630.10802098693460443No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC620.10630636809437263No Hit
AAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC620.10630636809437263No Hit
TTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC610.1045917492541408No Hit
CGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC610.1045917492541408No Hit
TGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC610.1045917492541408No Hit
CCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC610.1045917492541408No Hit
GAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC600.10287713041390899No Hit
TTAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC600.10287713041390899No Hit
ACACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC600.10287713041390899No Hit
CACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC600.10287713041390899No Hit
CGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC600.10287713041390899No Hit
TATCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC600.10287713041390899No Hit
ATGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC600.10287713041390899No Hit
ATAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC590.10116251157367717No Hit
GACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC590.10116251157367717No Hit
TCGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC590.10116251157367717No Hit
TTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC590.10116251157367717No Hit
TGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC590.10116251157367717No Hit
GGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC590.10116251157367717No Hit
TGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC590.10116251157367717No Hit
TTCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC590.10116251157367717No Hit
GAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC590.10116251157367717No Hit
ATTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC590.10116251157367717No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTCCT350.0295.000031
GCACCTA700.0295.000032
CCCCCTA850.0295.000032
GGCCTAC2950.0295.000033
GCTCCTA700.0295.000032
CCACCTA850.0295.000032
TGATCCT450.0295.01
TCGCCTA800.0295.02
GTTGATT400.0295.0295
GCCCCTA650.0295.02
TCGCCCT157.2052317E-6295.01
AGTCCTA1300.0295.02
AGCAAAT450.0295.0295
CTGTCCT206.1349056E-8295.01
GTCGCCT157.2052317E-6295.01
CCCCTAC3450.0295.03
CGAACCT206.1349056E-8295.01
ACGCCTA650.0295.02
ACGCCCT206.1349056E-8295.01
ATGGCCT400.0295.01
Produced by FastQC (version 0.11.5)