FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491850

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491850
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60861
Sequences flagged as poor quality0
Sequence length301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA33845.560210972543993No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA27824.571071786529961No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA22653.7215951101690736No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA20933.438983914165065No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA18683.069289035671448No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA18232.9953500599727247No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA17312.8441859318775573No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA16092.643729153316574No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA15042.4712048766862194No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA13952.292108246660423No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA13652.2428155961946072No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA13592.232957066101444No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA12862.113011616634626No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA11481.8862654244918748No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA11301.8566898342123856No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA11241.8468313041192224No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA10501.725242766303544No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA9941.6332298187673553No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA9821.6135127585810287No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA9191.5099981926028163No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA8981.4754933372767454No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA8681.4262006868109298No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA8041.3210430324838567No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA7851.289824353855507No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA7421.2191715548545046No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA7281.1961683179704574No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA7011.1518049325512232No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA6821.1205862539228735No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6131.0072131578514978No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA6080.9989977161071951No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6000.985853009316311No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5560.9135571219664481No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA5470.8987693268267035No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA5150.8461904996631668No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4980.818257997732538No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA4860.7985409375462118No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA4840.7952547608484909No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA4590.7541775521269779No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA4420.7262450501963491No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4010.6588784278930678No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA3750.6161581308226943No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA3620.5947979822875076No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA3510.5767240104500418No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3360.5520776852171341No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA3270.5372898900773895No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA3150.5175728298910632No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3130.5142866531933422No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2690.44199076584347946No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA2550.41898752895943214No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA2510.4124151755639901No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCACGCATTA2400.3943412037265244No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1980.3253314930743826No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1640.269466489213125No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1560.25632178242224085No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCACGCATTA1560.25632178242224085No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATCA1530.2513925173756593No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1470.2415339872824962No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCACGCATTA1310.2152445737007279No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1260.20702913195642528No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCACGCATTA1110.18238280672351753No Hit
GGACTACAAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA1090.17909663002579648No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCACGCATTA1000.16430883488605183No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCACGCATTA990.1626657465371913No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA990.1626657465371913No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCACGCATTA980.16102265818833078No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATCA900.14787795139744664No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCACGCATTA810.13309015625770196No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATCA800.13144706790884145No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCACGCATTA800.13144706790884145No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA800.13144706790884145No Hit
GGACTACACGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA780.12816089121112043No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA760.12487471451339939No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATCA740.12158853781567835No Hit
GGACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA700.11501618442023628No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA690.11337309607137576No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCACGCATTA690.11337309607137576No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCACGCATTA680.11173000772251523No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATCA670.11008691937365472No Hit
GGACTACCAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA620.10187147762935211No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA610.1002283892804916No Hit
GGACTACAGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA610.1002283892804916No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTGCC750.0295.0295
CGTTAGA206.13727E-8295.0295
TGGGGTA2300.0295.08
TGCCTAC108.4453734E-4295.01
TACTGGG2850.0295.05
TTTAGCA550.0295.0295
TGTAGCC206.13727E-8295.0295
TTTAGAC108.4453734E-4295.0295
GTTAGAC108.4453734E-4295.0295
CTACGGG108.4453734E-4295.04
ATGTAGA108.4453734E-4295.0295
AGGTAGA108.4453734E-4295.0295
ACTGGGG2850.0295.06
TCGGGTT900.0295.08
TTTAGTC157.2067833E-6294.99997295
GGACTAC58050.0294.74591
AAGGGTA9400.0293.430888
ACTACTA6600.0292.765173
TACTAGG6600.0292.765175
TACAAGG12400.0292.620975
Produced by FastQC (version 0.11.5)