FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491857

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491857
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79323
Sequences flagged as poor quality0
Sequence length301
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1040.13110951426446302No Hit
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1000.12606684063890675No Hit
TTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC990.12480617223251768No Hit
TACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC970.12228483541973954No Hit
ACATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC960.12102416701335048No Hit
TCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC960.12102416701335048No Hit
TAGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC930.11724216179418327No Hit
CTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC930.11724216179418327No Hit
ATCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC930.11724216179418327No Hit
TCTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC930.11724216179418327No Hit
ATAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC920.1159814933877942No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC920.1159814933877942No Hit
TCACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC910.11472082498140514No Hit
ATATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC910.11472082498140514No Hit
TTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC900.11346015657501607No Hit
ACCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC900.11346015657501607No Hit
TTTCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC890.112199488168627No Hit
ATGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC880.11093881976223793No Hit
ATCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC870.10967815135584887No Hit
CTACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC870.10967815135584887No Hit
TTACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC870.10967815135584887No Hit
CTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC870.10967815135584887No Hit
TTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC860.1084174829494598No Hit
CACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC860.1084174829494598No Hit
TTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC850.10715681454307073No Hit
CATTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC850.10715681454307073No Hit
CTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC840.10589614613668166No Hit
CAACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC830.1046354777302926No Hit
TCGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC830.1046354777302926No Hit
CACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC830.1046354777302926No Hit
TCCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC830.1046354777302926No Hit
CAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC830.1046354777302926No Hit
GTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC830.1046354777302926No Hit
GACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC820.10337480932390353No Hit
AATACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC820.10337480932390353No Hit
CTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC820.10337480932390353No Hit
ACAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC820.10337480932390353No Hit
ATGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC820.10337480932390353No Hit
TTGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC820.10337480932390353No Hit
GTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC820.10337480932390353No Hit
AAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC820.10337480932390353No Hit
CAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC810.10211414091751446No Hit
TCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC810.10211414091751446No Hit
AAGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC810.10211414091751446No Hit
TACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC810.10211414091751446No Hit
TAGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC800.10085347251112539No Hit
AACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC800.10085347251112539No Hit
TTAGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC800.10085347251112539No Hit
TATTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC800.10085347251112539No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCCT550.0295.000031
TGATCCT500.0295.01
TCGCCTA1300.0295.02
AATCCCT400.0295.01
GTCGCCT350.0295.01
AGATCCT255.275069E-10295.01
CGAACCT255.275069E-10295.01
ACGCCTA950.0295.02
ACGCCCT255.275069E-10295.01
CGGCCTA650.0295.02
AGCCCTA950.0295.02
TGGACCT350.0295.01
CCGGCCT108.450199E-4295.01
GGTCCTA950.0295.02
GTCTCCT350.0295.01
ATAGCCT400.0295.01
CGCGCCT206.148912E-8295.01
GCGCCTA1000.0295.02
CCTACGA255.275069E-10295.05
CCTACCG108.450199E-4295.05
Produced by FastQC (version 0.11.5)