FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491861

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491861
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69795
Sequences flagged as poor quality0
Sequence length301
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC950.13611290207034887No Hit
TCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC950.13611290207034887No Hit
CCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC930.13324736728992048No Hit
TTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC930.13324736728992048No Hit
TAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC910.1303818325094921No Hit
TCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC910.1303818325094921No Hit
GTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC910.1303818325094921No Hit
TTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC890.1275162977290637No Hit
CTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC870.12465076294863528No Hit
ATATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC850.12178522816820689No Hit
CAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC840.12035246077799269No Hit
TTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC830.1189196933877785No Hit
CACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC830.1189196933877785No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC830.1189196933877785No Hit
TTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC820.1174869259975643No Hit
ACAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC820.1174869259975643No Hit
ATCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC810.11605415860735009No Hit
TCTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC810.11605415860735009No Hit
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC800.1146213912171359No Hit
ATCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC800.1146213912171359No Hit
AACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC780.1117558564367075No Hit
CTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC780.1117558564367075No Hit
CTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC780.1117558564367075No Hit
ATGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC780.1117558564367075No Hit
AAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC780.1117558564367075No Hit
CTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC770.11032308904649331No Hit
ATAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC770.11032308904649331No Hit
ACCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC760.1088903216562791No Hit
GTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC760.1088903216562791No Hit
CCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC760.1088903216562791No Hit
TTGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC760.1088903216562791No Hit
TACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC760.1088903216562791No Hit
AAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC750.1074575542660649No Hit
TCTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC750.1074575542660649No Hit
AGCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC750.1074575542660649No Hit
TCACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC740.10602478687585071No Hit
CTGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC740.10602478687585071No Hit
TATACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC740.10602478687585071No Hit
CACCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC720.10315925209542232No Hit
ATTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC720.10315925209542232No Hit
TAACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC720.10315925209542232No Hit
CTCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC720.10315925209542232No Hit
CAACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC710.10172648470520812No Hit
GACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC710.10172648470520812No Hit
CTACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC710.10172648470520812No Hit
TATCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC710.10172648470520812No Hit
CTGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC710.10172648470520812No Hit
CATACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC710.10172648470520812No Hit
GTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC710.10172648470520812No Hit
TCGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.1002937173149939No Hit
TGTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.1002937173149939No Hit
TACCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.1002937173149939No Hit
ACATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.1002937173149939No Hit
AAGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.1002937173149939No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCCTA550.0295.000032
TGATCCT303.6379788E-12295.01
TCGCCTA1200.0295.02
GCGCGAA157.2113E-6295.0295
ACCGCCT157.2113E-6295.01
TACGCCT303.6379788E-12295.01
CGCTCCT303.6379788E-12295.01
CCGACCT303.6379788E-12295.01
CGGTAGG157.2113E-6295.09
TAAGCCT303.6379788E-12295.01
CGATCCT303.6379788E-12295.01
CCGCCTA850.0295.02
ATACCTA1400.0295.02
TCGCCCT108.448021E-4294.999971
ACGCCCT206.143637E-8294.999971
GCCATAA108.448021E-4294.99997295
GTGTCGT108.448021E-4294.99997295
GACGCCT108.448021E-4294.999971
TCGACCT400.0294.999971
ACCTACG18200.0293.37914
Produced by FastQC (version 0.11.5)