FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005491862

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491862
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69795
Sequences flagged as poor quality0
Sequence length301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA19902.8512071065262554No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA17872.560355326312773No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA13992.004441578909664No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA12961.8568665377176015No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA11481.6448169639659No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA11091.5889390357475461No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC9681.3869188337273444No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA9141.3095493946557777No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA8971.2851923490221362No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC7711.1046636578551472No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA7561.0831721470019342No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA7361.0545167991976503No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC6780.9714162905652267No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA6040.865391503689376No Hit
GGACTACTAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG5620.8052152733003797No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA5420.7765599254960958No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC5330.7636650189841678No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA5260.7536356472526685No Hit
GGACTACAAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG4980.713518160326671No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4970.7120853929364568No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4970.7120853929364568No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4940.7077870907658141No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA4870.6977577190343148No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA4320.6189555125725338No Hit
GGACTACTCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG4150.5945984669388925No Hit
GGACTACTGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC4060.5817035604269647No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3780.5415860735009671No Hit
GGACTACCCGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC3700.5301239343792535No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3600.5157962604771116No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA3420.490006447453256No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA3360.48140984311197077No Hit
GGACTACACGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG3270.468514936600043No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA3260.4670821692098288No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA3210.4599183322587578No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA3020.432695751844688No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2880.41263700838168926No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2830.40547317143061823No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2800.4011748692599756No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA2750.3940110323089046No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA2740.39257826491869047No Hit
GGACTACTAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2610.37395228884590587No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA2490.3567590801633355No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2440.3495952432122645No Hit
GGACTACTGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2360.3381331040905509No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2270.3252381975786231No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2260.3238054301884089No Hit
GGACTACCCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2250.3223726627981947No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2150.30804498889605275No Hit
GGACTACCGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2140.30661222150583856No Hit
GGACTACCAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2090.2994483845547675No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2040.29228454760369654No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA2030.29085178021348235No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1920.27509133892112614No Hit
GGACTACAAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1860.26649473457984096No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA1800.2578981302385558No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1790.25646536284834154No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1760.252167060677699No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1730.2478687585070564No Hit
GGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA1710.245003223726628No Hit
GGACTACTAGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA1690.24213768894619955No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1610.23067554982448601No Hit
GGACTACTAGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1600.2292427824342718No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA1560.223511712873415No Hit
GGACTACAAGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1530.2192134107027724No Hit
GGACTACAAGGGTTTCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA1530.2192134107027724No Hit
GGACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1480.21204957375170141No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA1460.20918403897127302No Hit
GGACTACTCGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1450.2077512715810588No Hit
GGACTACCGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1370.19628913245934523No Hit
GGACTACACGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1340.19199083028870265No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1320.18912529550827423No Hit
GGACTACTAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1300.18625976072784584No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1290.18482699333763164No Hit
GGACTACTCGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1280.18339422594741744No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA1250.17909592377677483No Hit
GGACTACAAGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA1240.17766315638656063No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1230.17623038899634644No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1220.17479762160613224No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA1210.17336485421591805No Hit
GGACTACAAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1210.17336485421591805No Hit
GGACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1190.17049931943548965No Hit
GGACTACAGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1190.17049931943548965No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1150.16476824987463284No Hit
GGACTACTCGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA1150.16476824987463284No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1130.16190271509420445No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA1120.16046994770399026No Hit
GGACTACACGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1090.15617164553334764No Hit
GGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA1070.15330611075291928No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA1040.14900780858227666No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1010.14470950641163408No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA980.14041120424099146No Hit
GGACTACTGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT960.13754566946056307No Hit
GGACTACCAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG930.13324736728992048No Hit
GGACTACTAGGGTTTCTAATACCTCTTGTCCATGTCTTATGATACGGCCA920.1318145998997063No Hit
GGACTACAAGGGTTTCTAAAAGGCTAGATAGTAACTGTGGAATTTTGCAA900.1289490651192779No Hit
GGACTACTAGGGTTTCTAAAAGGCTAGATAGTAACTGTGGAATTTTGCAA890.1275162977290637No Hit
GGACTACACGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA890.1275162977290637No Hit
GGACTACTCGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG890.1275162977290637No Hit
GGACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC870.12465076294863528No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA840.12035246077799269No Hit
GGACTACCCGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT840.12035246077799269No Hit
GGACTACACGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG810.11605415860735009No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC810.11605415860735009No Hit
GGACTACAAGGGTTTCTAATACCTCTTGTCCATGTCTTATGATACGGCCA800.1146213912171359No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA790.1131886238269217No Hit
GGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA760.1088903216562791No Hit
GGACTACTCGGGTTTCTAAAAGGCTAGATAGTAACTGTGGAATTTTGCAA740.10602478687585071No Hit
GGACTACTGGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA740.10602478687585071No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA730.10459201948563651No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA730.10459201948563651No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA710.10172648470520812No Hit
GGACTACTAGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGTGCCTCA700.1002937173149939No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)