FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491865

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491865
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59663
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1080.18101671052411042No Hit
GTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC990.1659319846471012No Hit
ATATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC980.1642559039941002No Hit
TAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC970.1625798233410992No Hit
CCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC970.1625798233410992No Hit
TTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC940.1575515813820961No Hit
TTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC940.1575515813820961No Hit
CTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC930.1558755007290951No Hit
ACAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC900.150847258770092No Hit
TCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC900.150847258770092No Hit
CCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC890.149171178117091No Hit
TCGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC890.149171178117091No Hit
ATGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC860.14414293615808793No Hit
ATACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC840.14079077485208588No Hit
ATTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC840.14079077485208588No Hit
TACCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC840.14079077485208588No Hit
ACATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC830.13911469419908487No Hit
TACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC830.13911469419908487No Hit
TTGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC820.13743861354608383No Hit
TCTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC820.13743861354608383No Hit
TATACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC820.13743861354608383No Hit
TTAGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC810.13576253289308282No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC810.13576253289308282No Hit
TACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC800.13408645224008178No Hit
CGCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC790.13241037158708077No Hit
CTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC790.13241037158708077No Hit
ATCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC780.13073429093407976No Hit
TCCGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC780.13073429093407976No Hit
ATCGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC770.12905821028107872No Hit
TAGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC770.12905821028107872No Hit
TGCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC770.12905821028107872No Hit
TTTCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC770.12905821028107872No Hit
ATCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC770.12905821028107872No Hit
CCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC770.12905821028107872No Hit
TCACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC760.1273821296280777No Hit
CAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC760.1273821296280777No Hit
TAAGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC760.1273821296280777No Hit
TCAGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC760.1273821296280777No Hit
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC760.1273821296280777No Hit
TCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC750.12570604897507667No Hit
CTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC750.12570604897507667No Hit
TAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC740.12402996832207565No Hit
AGCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC740.12402996832207565No Hit
AGGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC740.12402996832207565No Hit
CCCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC740.12402996832207565No Hit
CTCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC740.12402996832207565No Hit
CATTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC730.12235388766907464No Hit
TCGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC730.12235388766907464No Hit
GTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC720.12067780701607361No Hit
ATAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC720.12067780701607361No Hit
CAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC720.12067780701607361No Hit
CTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC720.12067780701607361No Hit
AACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC720.12067780701607361No Hit
TTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC720.12067780701607361No Hit
CGGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC710.1190017263630726No Hit
TCTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC710.1190017263630726No Hit
AAGCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC710.1190017263630726No Hit
GTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC710.1190017263630726No Hit
GGCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC710.1190017263630726No Hit
GTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC710.1190017263630726No Hit
AAGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC710.1190017263630726No Hit
AAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC710.1190017263630726No Hit
CTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.11732564571007156No Hit
ATCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.11732564571007156No Hit
CTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.11732564571007156No Hit
CCGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.11732564571007156No Hit
CGTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC690.11564956505707054No Hit
TCCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC690.11564956505707054No Hit
CCCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC690.11564956505707054No Hit
CACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC690.11564956505707054No Hit
TTCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC690.11564956505707054No Hit
AAACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC680.11397348440406953No Hit
CACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC680.11397348440406953No Hit
CCTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC680.11397348440406953No Hit
AGAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC680.11397348440406953No Hit
TCTCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC680.11397348440406953No Hit
AAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC680.11397348440406953No Hit
CTACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC670.1122974037510685No Hit
ACCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC670.1122974037510685No Hit
CCAGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC670.1122974037510685No Hit
AATTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC660.11062132309806749No Hit
AACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC660.11062132309806749No Hit
TTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC660.11062132309806749No Hit
ACGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC660.11062132309806749No Hit
CAGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC660.11062132309806749No Hit
ATTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC660.11062132309806749No Hit
TCGCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC660.11062132309806749No Hit
CACCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC660.11062132309806749No Hit
ATTCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC660.11062132309806749No Hit
GATTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC660.11062132309806749No Hit
ACACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC650.10894524244506645No Hit
ATGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC650.10894524244506645No Hit
TGCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC650.10894524244506645No Hit
TGGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC650.10894524244506645No Hit
GGATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.10726916179206543No Hit
CCTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.10726916179206543No Hit
CGAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.10726916179206543No Hit
CAACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.10726916179206543No Hit
GACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.10726916179206543No Hit
AAAGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.10726916179206543No Hit
GTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.10726916179206543No Hit
CCGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.10726916179206543No Hit
GGGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC630.10559308113906442No Hit
TTACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC630.10559308113906442No Hit
AGCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC630.10559308113906442No Hit
GCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC630.10559308113906442No Hit
CGATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC630.10559308113906442No Hit
TGTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC630.10559308113906442No Hit
AGATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC630.10559308113906442No Hit
AGTCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC630.10559308113906442No Hit
CTAGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC620.1039170004860634No Hit
CTGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC620.1039170004860634No Hit
CAGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC620.1039170004860634No Hit
TAACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC620.1039170004860634No Hit
GGTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC620.1039170004860634No Hit
TTGCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC620.1039170004860634No Hit
GAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC620.1039170004860634No Hit
GCACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC610.10224091983306235No Hit
GAGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC610.10224091983306235No Hit
CAAGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC610.10224091983306235No Hit
CATACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC610.10224091983306235No Hit
CCACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC610.10224091983306235No Hit
GTGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC610.10224091983306235No Hit
CTTCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC610.10224091983306235No Hit
GCTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC610.10224091983306235No Hit
TGGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC610.10224091983306235No Hit
TAGGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC610.10224091983306235No Hit
GTCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC600.10056483918006134No Hit
GCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC600.10056483918006134No Hit
CGACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC600.10056483918006134No Hit
CATCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC600.10056483918006134No Hit
GTGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC600.10056483918006134No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACCTA850.0295.000032
GGACCTA850.0295.000032
TCGCCCT303.6379788E-12295.01
GTACCTA1000.0295.02
GTACCCT157.2060684E-6295.01
TGCCCCT206.136179E-8295.01
CGAACCT206.136179E-8295.01
CCCTCGG108.444956E-4295.04
GTTTCCT206.136179E-8295.01
CACGGAG157.2060684E-6295.06
CGGCCCT108.444956E-4295.01
ACACCTA1550.0295.02
CCGGCCT255.2568794E-10295.01
GATCCTA900.0295.02
GGTCCTA1200.0295.02
CGCGCCT108.444956E-4295.01
GCGCCTA700.0295.02
CCTACGA157.2060684E-6295.05
TCCTCGG108.444956E-4295.04
GTCTACG108.444956E-4295.03
Produced by FastQC (version 0.11.5)