FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491867

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491867
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81574
Sequences flagged as poor quality0
Sequence length301
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCCTA1100.0295.000032
TGATCCT255.275069E-10295.01
GTTGATT206.150003E-8295.0295
TCTGCCT206.150003E-8295.01
TCGCCCT350.0295.01
ACAACCT450.0295.01
AGTCCTA900.0295.02
GTCGCCT206.150003E-8295.01
AGATCCT255.275069E-10295.01
CGAACCT350.0295.01
ACGCCCT303.6379788E-12295.01
GCGACCT400.0295.01
TCCGCCT255.275069E-10295.01
GGGTCCT108.450638E-4295.01
GGCACCT350.0295.01
ACCGCCT450.0295.01
GCAACCT400.0295.01
TTGTCCT400.0295.01
GATCCCT206.150003E-8295.01
CAACCTA1400.0295.02
Produced by FastQC (version 0.11.5)