FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491872

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491872
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62055
Sequences flagged as poor quality0
Sequence length301
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA37286.007573926355652No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA27244.3896543388929175No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA21553.472725807751188No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA17622.8394166465232455No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA16502.658931592941745No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA16122.5976955926194507No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA15652.5219563290629283No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA15002.41721053903795No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA13522.1787124325195393No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA12181.9627749576988156No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA11171.800016114736927No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA9941.6018048505358151No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA7951.2811215856901137No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA7761.2505035855289663No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA7451.2005479010555153No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA7421.1957134799774394No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA7371.1876561115139797No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA6781.0925791636451534No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6160.9926677946982515No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5540.8927564257513496No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5430.8750302151317381No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5230.8428007412778986No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4990.8041253726532913No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4770.7686729514140682No Hit
GGACTACAAGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA4380.7058254773990815No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3880.6252517927644832No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3800.6123600032229474No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3740.6026911610667955No Hit
GGACTACTAGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA3360.5414551607445008No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3310.533397792281041No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3250.5237289501248892No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2940.47377326565143824No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2780.4479896865683668No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2720.438320844412215No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA2610.4205946337926033No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2590.4173716864072194No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2520.40609137055837563No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2500.40286842317299165No Hit
GGACTACACGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA2300.37063894931915237No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA2060.3319635806945451No Hit
GGACTACTCGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1920.30940294899685766No Hit
GGACTACCAGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1910.3077914753041657No Hit
GGACTACAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1880.30295705422608976No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1830.2948996857626299No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1800.290065264684554No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1780.2868423172991701No Hit
GGACTACTGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1650.26589315929417456No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1610.25944726452340666No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1610.25944726452340666No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1550.24977842236725487No Hit
GGACTACAAGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1530.24655547498187094No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1470.23688663282571912No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA1460.23527515913302713No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1440.2320522117476432No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1420.22882926436225925No Hit
GGACTACTAGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1410.22721779066956732No Hit
GGACTACCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1400.2256063169768754No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1310.21110305374264765No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1290.2078801063572637No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1260.20304568527918782No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1210.19498831681572798No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1200.19337684312303602No Hit
GGACTACCCGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1200.19337684312303602No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1160.18693094835226814No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1150.18531947465957618No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1130.18209652727419226No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA1100.17726210619611635No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1080.1740391588107324No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1040.16759326403996455No Hit
GGACTACTCGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1030.16598179034727256No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1020.1643703166545806No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1000.16114736926919668No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1000.16114736926919668No Hit
GGACTACACGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG990.15953589557650472No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC980.15792442188381275No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC970.1563129481911208No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA950.15309000080573684No Hit
GGACTACAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG910.146644106034969No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA910.146644106034969No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA870.14019821126420112No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA860.13858673757150913No Hit
GGACTACTGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG850.13697526387881717No Hit
GGACTACAAGGTTTCTAATCCTGTTTGATACCCCCACTTTCGAGCCTCAA840.1353637901861252No Hit
GGACTACAAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT840.1353637901861252No Hit
GGACTACCAGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG780.1256949480299734No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA780.1256949480299734No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA750.1208605269518975No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA740.11924905325920554No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA730.11763757956651356No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA730.11763757956651356No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA720.1160261058738216No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA710.11441463218112963No Hit
GGACTACCCGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG710.11441463218112963No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA690.11119168479574572No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA690.11119168479574572No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA680.10958021110305376No Hit
GGACTACCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG680.10958021110305376No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA670.10796873741036178No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA670.10796873741036178No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC660.10635726371766982No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA660.10635726371766982No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA660.10635726371766982No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA630.10152284263959391No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA630.10152284263959391No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACTAT108.425461E-4295.23711
GGACTAC57700.0295.23711
ACACGGT900.0294.99926
CTACTAG8300.0294.99924
CACGGTT900.0294.99927
GGGGTAT750.0294.99929
TACTGGG5400.0294.99925
AGCTAGC108.4458437E-4294.9992295
TACACGG7200.0294.99925
ACAAGGT1700.0294.99926
ACAAGGG10800.0294.99926
GGTAGCC157.2075454E-6294.9992295
TACAAGG12500.0294.99925
ACGGTTT900.0294.99928
CGTTAGC350.0294.99918295
CAAGGTT1650.0294.999187
ACCCGGT500.0294.999186
GACTACA23750.0294.378142
CTACAAG12550.0293.823884
GGGGTTT11400.0293.705329
Produced by FastQC (version 0.11.5)