FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491877

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491877
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50296
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT660.13122315889931604No Hit
CTTACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT640.12724669953873072No Hit
ATATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT630.12525846985843803No Hit
TCTTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT630.12525846985843803No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC620.12327024017814538No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT610.1212820104978527No Hit
CACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC590.11730555113726737No Hit
CTATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT580.11531732145697471No Hit
ACTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC580.11531732145697471No Hit
TTAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT570.11332909177668203No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC560.11134086209638938No Hit
ATCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT550.1093526324160967No Hit
TCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC550.1093526324160967No Hit
AAATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT550.1093526324160967No Hit
TTCTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT540.10736440273580404No Hit
TCCTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT540.10736440273580404No Hit
TAAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT540.10736440273580404No Hit
CACACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT530.10537617305551136No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC520.10338794337521871No Hit
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT520.10338794337521871No Hit
CCCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT520.10338794337521871No Hit
TTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC510.10139971369492604No Hit
TCAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT510.10139971369492604No Hit
AAACCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT510.10139971369492604No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCCCT550.0295.000031
GTCACCT350.0295.000031
TGACCTA1100.0295.000032
CTCCCTA700.0295.000032
AGGGCCT350.0295.000031
TAGTCCT350.0295.000031
CTTACCT350.0295.000031
TTGACCT350.0295.000031
CCCTCCT350.0295.000031
CCGACCT350.0295.000031
ACAACCT550.0295.000031
GTGCCTA550.0295.000032
CAGCCCT108.4409985E-4295.01
CGTTCCT206.126538E-8295.01
ACAGCCT255.2386895E-10295.01
TTGTCCT157.1993163E-6295.01
TCCTCGG108.4409985E-4295.04
CCTACTG108.4409985E-4295.05
CCTACCT108.4409985E-4295.01
ATCTCCT206.126538E-8295.01
Produced by FastQC (version 0.11.5)