FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491879

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491879
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68727
Sequences flagged as poor quality0
Sequence length301
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT1210.17605889970462846No Hit
CTCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT1090.15859851295706198No Hit
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT1090.15859851295706198No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT1080.1571434807280981No Hit
ATCTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT1080.1571434807280981No Hit
TTCCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT1020.14841328735431492No Hit
CTAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT1020.14841328735431492No Hit
CACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT1010.14695825512535102No Hit
TAATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT1010.14695825512535102No Hit
ACCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT1000.14550322289638715No Hit
CAATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT1000.14550322289638715No Hit
TTACCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT990.14404819066742328No Hit
CCCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT990.14404819066742328No Hit
TAAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT990.14404819066742328No Hit
TTTACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT980.1425931584384594No Hit
TACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT970.14113812620949556No Hit
CTATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT970.14113812620949556No Hit
ATCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT960.13968309398053166No Hit
ACCTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT960.13968309398053166No Hit
TGGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT960.13968309398053166No Hit
CAAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT960.13968309398053166No Hit
ATGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT940.13677302952260392No Hit
GACACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT940.13677302952260392No Hit
TTAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT940.13677302952260392No Hit
ATTTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT930.13531799729364005No Hit
ACATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT930.13531799729364005No Hit
CATTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT930.13531799729364005No Hit
CTCTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT930.13531799729364005No Hit
GTTTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT930.13531799729364005No Hit
ACAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT930.13531799729364005No Hit
AAATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT920.13386296506467618No Hit
TCCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT920.13386296506467618No Hit
TCGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT920.13386296506467618No Hit
CTTTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT910.1324079328357123No Hit
TTTTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT900.13095290060674844No Hit
CCAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT900.13095290060674844No Hit
ATAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT900.13095290060674844No Hit
CCTTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT900.13095290060674844No Hit
TCCTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT900.13095290060674844No Hit
ATTACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT900.13095290060674844No Hit
TTGACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT890.12949786837778457No Hit
TCACCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT890.12949786837778457No Hit
ATACCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT890.12949786837778457No Hit
ATATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT880.1280428361488207No Hit
CACACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT880.1280428361488207No Hit
AACACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT880.1280428361488207No Hit
TTCTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT870.12658780391985683No Hit
GTAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT870.12658780391985683No Hit
ATGACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT870.12658780391985683No Hit
TCAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT860.12513277169089296No Hit
GGATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT860.12513277169089296No Hit
GTCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT860.12513277169089296No Hit
ATCCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT850.12367773946192909No Hit
TCTTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT850.12367773946192909No Hit
GTATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT840.1222227072329652No Hit
GTGCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT840.1222227072329652No Hit
CGTTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT840.1222227072329652No Hit
TACACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT830.12076767500400133No Hit
CTTACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT830.12076767500400133No Hit
GAATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT830.12076767500400133No Hit
CCTACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT830.12076767500400133No Hit
AGGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT820.11931264277503748No Hit
TGATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT820.11931264277503748No Hit
CATACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT810.1178576105460736No Hit
AAAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT800.11640257831710972No Hit
TGAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT800.11640257831710972No Hit
CCACCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT790.11494754608814585No Hit
ACCCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT790.11494754608814585No Hit
GTCTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT790.11494754608814585No Hit
GTGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT790.11494754608814585No Hit
GCTTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT780.11349251385918198No Hit
TCGACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT780.11349251385918198No Hit
CGCTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT780.11349251385918198No Hit
ACGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT770.11203748163021812No Hit
TAGACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT770.11203748163021812No Hit
AACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT770.11203748163021812No Hit
TACGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT770.11203748163021812No Hit
TTCGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT760.11058244940125422No Hit
ACACCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT760.11058244940125422No Hit
CACCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT760.11058244940125422No Hit
AAACCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT760.11058244940125422No Hit
CCCTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT760.11058244940125422No Hit
TTTGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT750.10912741717229037No Hit
GGTTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT750.10912741717229037No Hit
CGGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT750.10912741717229037No Hit
ATTCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT750.10912741717229037No Hit
AGATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT740.1076723849433265No Hit
GTTCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT740.1076723849433265No Hit
CCGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT740.1076723849433265No Hit
CAACCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT740.1076723849433265No Hit
CATCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT740.1076723849433265No Hit
TAGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT740.1076723849433265No Hit
CTGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT740.1076723849433265No Hit
AAGACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT740.1076723849433265No Hit
GGACCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT730.10621735271436263No Hit
CCATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT730.10621735271436263No Hit
GGGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT730.10621735271436263No Hit
TTAGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT730.10621735271436263No Hit
GTTACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT730.10621735271436263No Hit
GAGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT720.10476232048539874No Hit
TGCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT720.10476232048539874No Hit
CTAGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT720.10476232048539874No Hit
CCGACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT720.10476232048539874No Hit
TTTCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT720.10476232048539874No Hit
TCTACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT710.10330728825643487No Hit
GTCCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT710.10330728825643487No Hit
TTGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT710.10330728825643487No Hit
AACCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT710.10330728825643487No Hit
GCAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT710.10330728825643487No Hit
ACGGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT700.10185225602747101No Hit
AATACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT700.10185225602747101No Hit
AATCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT700.10185225602747101No Hit
CTGCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT700.10185225602747101No Hit
AGCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT700.10185225602747101No Hit
ACTTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT700.10185225602747101No Hit
CAAGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT700.10185225602747101No Hit
CGACCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT700.10185225602747101No Hit
TCGCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT700.10185225602747101No Hit
TGGACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT700.10185225602747101No Hit
ATCGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT700.10185225602747101No Hit
ACGCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT700.10185225602747101No Hit
AGAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT700.10185225602747101No Hit
TGCTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT700.10185225602747101No Hit
GGGACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT690.10039722379850714No Hit
ATTGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT690.10039722379850714No Hit
CTCGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT690.10039722379850714No Hit
AGTACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT690.10039722379850714No Hit
TATCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT690.10039722379850714No Hit
AGTTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT690.10039722379850714No Hit
GCATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT690.10039722379850714No Hit
ACTACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT690.10039722379850714No Hit
TGTTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT690.10039722379850714No Hit
GGAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT690.10039722379850714No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCCT350.0295.000031
TAGACCT350.0295.000031
ATTACCT350.0295.000031
GGATCCT350.0295.000031
GACCCCT350.0295.000031
GTTACCT350.0295.000031
TATTCCT350.0295.000031
ATCCCCT350.0295.000031
CATACCT350.0295.000031
GATTCCT350.0295.000031
CTCTCCT350.0295.000031
TTCGCCT350.0295.000031
CGCCCTA700.0295.000032
CTTACCT350.0295.000031
GTTGATT303.6379788E-12295.0295
GCCCCTA850.0295.02
AGTCCTA1350.0295.02
ATCTCCT303.6379788E-12295.01
GTCGCCT157.2108287E-6295.01
CCCCTCG157.2108287E-6295.03
Produced by FastQC (version 0.11.5)