FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491883

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491883
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81382
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1290.1585117102061881No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1280.1572829372588533No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1200.14745275368017496No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1200.14745275368017496No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1180.1449952077855054No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1170.14376643483817061No Hit
TATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1160.1425376618908358No Hit
TTCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1140.14008011599616624No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1130.13885134304883143No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1100.13516502420682705No Hit
CTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1100.13516502420682705No Hit
ATCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1100.13516502420682705No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1090.13393625125949227No Hit
TTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1070.1314787053648227No Hit
ATTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1070.1314787053648227No Hit
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1050.1290211594701531No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1020.12533484062814873No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1010.12410606768081395No Hit
CAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1000.12287729473347915No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1000.12287729473347915No Hit
AAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1000.12287729473347915No Hit
CACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1000.12287729473347915No Hit
CAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1000.12287729473347915No Hit
TGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC990.12164852178614435No Hit
GTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC990.12164852178614435No Hit
AAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC990.12164852178614435No Hit
CATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC990.12164852178614435No Hit
TTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC980.12041974883880956No Hit
CTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC980.12041974883880956No Hit
TCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC980.12041974883880956No Hit
TGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC970.11919097589147477No Hit
ACAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC970.11919097589147477No Hit
GTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC960.11796220294413998No Hit
AATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC960.11796220294413998No Hit
TATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC960.11796220294413998No Hit
TTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC950.11673342999680518No Hit
TCGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC950.11673342999680518No Hit
CCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC950.11673342999680518No Hit
GTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC940.11550465704947041No Hit
CCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC940.11550465704947041No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC940.11550465704947041No Hit
GGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC940.11550465704947041No Hit
TCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC930.11427588410213561No Hit
CACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC930.11427588410213561No Hit
CTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC930.11427588410213561No Hit
TCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC930.11427588410213561No Hit
ATCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC930.11427588410213561No Hit
ACTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC930.11427588410213561No Hit
GTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC920.11304711115480082No Hit
CCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC920.11304711115480082No Hit
TAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC920.11304711115480082No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC920.11304711115480082No Hit
TAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC920.11304711115480082No Hit
CTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC910.11181833820746603No Hit
ACACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC910.11181833820746603No Hit
CCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC910.11181833820746603No Hit
TCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC910.11181833820746603No Hit
TTTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC910.11181833820746603No Hit
CTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC900.11058956526013124No Hit
ATTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC900.11058956526013124No Hit
CAGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC900.11058956526013124No Hit
GGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC890.10936079231279644No Hit
CCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC880.10813201936546164No Hit
TGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC880.10813201936546164No Hit
TACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC880.10813201936546164No Hit
TTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC880.10813201936546164No Hit
CCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC870.10690324641812686No Hit
CAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC870.10690324641812686No Hit
AACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC870.10690324641812686No Hit
TTCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC870.10690324641812686No Hit
TAGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC860.10567447347079206No Hit
AATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC860.10567447347079206No Hit
GGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC850.10444570052345727No Hit
TCCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC850.10444570052345727No Hit
CCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC850.10444570052345727No Hit
TAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC850.10444570052345727No Hit
TGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC850.10444570052345727No Hit
TGACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC850.10444570052345727No Hit
AAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC840.1032169275761225No Hit
GCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC840.1032169275761225No Hit
CATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC840.1032169275761225No Hit
CGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC840.1032169275761225No Hit
GCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC840.1032169275761225No Hit
TCGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC840.1032169275761225No Hit
CGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC830.1019881546287877No Hit
CTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC820.1007593816814529No Hit
AGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC820.1007593816814529No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGATT550.0295.00003295
ACGCCTA950.0295.000032
GGACCTA1100.0295.000032
TGATCCT400.0295.01
TCGCCTA1600.0295.02
CGGCGGC108.450601E-4295.09
AGCAAAT108.450601E-4295.0295
GAAGCCT350.0295.01
GGAGCCT255.275069E-10295.01
CGAACCT650.0295.01
CAGCCTA1150.0295.02
CAGCCCT255.275069E-10295.01
GATCCTA1150.0295.02
TTGTCCT700.0295.01
GATCCCT255.275069E-10295.01
CACGCCT108.450601E-4295.01
CGCGCCT206.149821E-8295.01
CCTACGA206.149821E-8295.05
CCTACCT255.275069E-10295.01
CATCTAC108.450601E-4295.02
Produced by FastQC (version 0.11.5)