FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491887

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491887
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71166
Sequences flagged as poor quality0
Sequence length301
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCCTACGGGAGGCAGCAGGACTAGGGGGTTGGGGACAGATTTTATTG730.10257707332152995No Hit
TTTACCTACGGGAGGCAGCAGGACTAGGGGGTTGGGGACAGATTTTATTG720.10117190793356377No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAA108.448375E-4295.00003295
CGCGCCT108.448375E-4295.000031
CCGACCT108.448375E-4295.000031
GGCGCCT206.144546E-8295.000031
GCGCCTA550.0295.02
GGGCCTA650.0295.02
AGATCCT157.211909E-6294.999971
CGCTCCT600.0294.999971
GAACCTA1050.0294.999972
GTTGCCT157.211909E-6294.999971
ACCTACG20150.0293.5364
CCTACGG65750.0291.410685
CTACGGG64350.0291.103336
ACCCTAC3750.0291.066653
GCCTACG12600.0290.317444
ACGGGAG63750.0290.141178
TCCTACG18450.0289.40384
GACCTAC4500.0288.444463
CCCTACG14900.0288.070474
TACGGGA64350.0287.894327
Produced by FastQC (version 0.11.5)