FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491889

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491889
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69173
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTCG108.447863E-4295.03
GTACCTA600.0295.02
GTACCCT157.2110324E-6295.01
ACGCCTA800.0295.02
CGCGCCT255.2568794E-10295.01
GCGCCCT157.2110324E-6295.01
GTCTACG108.447863E-4295.03
CGTTCCT400.0295.01
GCACCCT255.2568794E-10295.01
CACCTAG108.447863E-4295.03
TACGCCT255.2568794E-10295.01
GGGACCT206.143273E-8295.01
CCCCCTA850.0295.02
CGAGCCT157.2110324E-6295.01
GGACCTA1100.0295.02
CGGTCCT157.2110324E-6295.01
GGCGCCT255.2568794E-10295.01
CGCACCT157.2110324E-6295.01
CGATCCT206.143273E-8295.01
ATCGCCT303.6379788E-12295.01
Produced by FastQC (version 0.11.5)