FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491893

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491893
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117101
Sequences flagged as poor quality0
Sequence length301
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1320.11272320475486973No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1210.10332960435863058No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1200.1024756406862452No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1200.1024756406862452No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1190.10162167701385982No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCT850.0295.01
GTTGATT255.275069E-10295.0295
AGTCCTC108.455339E-4295.02
ATCTCCT400.0295.01
GTCGCCT206.161281E-8295.01
ACGCCTA1800.0295.02
GCGACCT400.0295.01
TCACCCT500.0295.01
CTTCCCT500.0295.01
CCTAGGG450.0295.05
CCTAGGA108.455339E-4295.05
CTATCCT750.0295.01
CCCTAGG108.455339E-4295.04
TAGACCT550.0295.01
GTCCTAC8750.0295.03
GATCCTA2400.0295.02
ATTACCT450.0295.01
CTCCCTA1450.0295.02
GGTCCTA1850.0295.02
TTGTCCT750.0295.01
Produced by FastQC (version 0.11.5)