FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491899

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491899
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73007
Sequences flagged as poor quality0
Sequence length301
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGCAAGTC800.10957853356527456No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCCCT303.6379788E-12295.01
ATCCCCT303.6379788E-12295.01
ACAGCCT157.212677E-6295.01
ATCCTAG157.212677E-6295.03
AGCCTAC3150.0295.03
GTTGATT350.0295.0295
TGGTCCT255.2568794E-10295.01
TGCACCT255.2568794E-10295.01
GCCTCCT350.0295.01
CCTACGA303.6379788E-12295.05
GTCACCT157.212677E-6295.01
CCTACCT400.0295.01
GGCACCT450.0295.01
ATCTCCT157.212677E-6295.01
TCGACCT157.212677E-6295.01
CGCCCTA950.0295.02
AAATCCT400.0295.01
TTTCCTA1350.0295.02
TTCGCCT255.2568794E-10295.01
TGACCCT255.2568794E-10295.01
Produced by FastQC (version 0.11.5)