FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491907

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491907
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112192
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1150.1025028522532801No Hit
TCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1150.1025028522532801No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1140.10161152310325158No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCCTA1250.0295.000032
GCATAAG108.4548636E-4295.04
TCGCCTA1850.0295.02
CGGCGGC108.4548636E-4295.08
TTGGCCT450.0295.01
GATCCTA2200.0295.02
GGTCCGG108.4548636E-4295.0295
CGCGCCT255.275069E-10295.01
CCTACGA305.456968E-12295.05
CGTTCCT500.0295.01
GACCCCT400.0295.01
ACCTCCT305.456968E-12295.01
GTAGCCT255.275069E-10295.01
TCGGAAA108.4548636E-4295.0295
AGACCCT255.275069E-10295.01
CCCCCCT450.0295.01
GTTCCCT255.275069E-10295.01
CTCGCCT600.0295.01
GCCTAGG157.2229886E-6295.04
CCGACCT305.456968E-12295.01
Produced by FastQC (version 0.11.5)