FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491909

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491909
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72805
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA950.0295.000032
CGAACCT255.2568794E-10295.01
ACGCCTA1350.0295.02
ACGCCCT157.2125968E-6295.01
GATCCTA1450.0295.02
CGACCTC108.448778E-4295.0295
CCTACGC108.448778E-4295.05
CCTACGA108.448778E-4295.05
GCACCCT350.0295.01
CGCCTAC3700.0295.03
ATTCCCT255.2568794E-10295.01
CGGGGCA206.145456E-8295.09
AATGCCT108.448778E-4295.01
CGCTCCT350.0295.01
CTACGAG108.448778E-4295.06
CTGCCTA650.0295.02
GGCGCCT157.2125968E-6295.01
CGTCCTA800.0295.02
TTCGCCT303.6379788E-12295.01
CGATCCT350.0295.01
Produced by FastQC (version 0.11.5)