FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005491915

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491915
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69240
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC1370.1978625072212594No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC1370.1978625072212594No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC1300.18775274407856732No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC1280.18486424032351242No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC1180.170421721548238No Hit
CACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC1160.16753321779318311No Hit
TTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC1160.16753321779318311No Hit
TAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC1160.16753321779318311No Hit
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC1140.16464471403812825No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC1100.1588677065280185No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC1080.1559792027729636No Hit
CCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC1070.15453495089543617No Hit
TCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC1060.15309069901790873No Hit
CTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC1050.15164644714038128No Hit
CCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC1040.15020219526285383No Hit
GAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC1030.1487579433853264No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC1030.1487579433853264No Hit
CATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC1020.14731369150779897No Hit
TCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC1020.14731369150779897No Hit
GTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC1020.14731369150779897No Hit
CAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC1020.14731369150779897No Hit
CCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC1020.14731369150779897No Hit
TACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC1010.14586943963027152No Hit
ATCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC1010.14586943963027152No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC1010.14586943963027152No Hit
CATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC1000.14442518775274407No Hit
CAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC1000.14442518775274407No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC1000.14442518775274407No Hit
CACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC1000.14442518775274407No Hit
AAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC990.14298093587521665No Hit
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC990.14298093587521665No Hit
ACAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC980.1415366839976892No Hit
ATCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC980.1415366839976892No Hit
TGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC980.1415366839976892No Hit
AACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC970.14009243212016176No Hit
TATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC970.14009243212016176No Hit
TGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC970.14009243212016176No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC970.14009243212016176No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC960.1386481802426343No Hit
CCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC960.1386481802426343No Hit
TTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC950.1372039283651069No Hit
GTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC950.1372039283651069No Hit
ACCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC950.1372039283651069No Hit
CTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC950.1372039283651069No Hit
TCCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC940.13575967648757944No Hit
ATTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC940.13575967648757944No Hit
AAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC940.13575967648757944No Hit
CGTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC930.134315424610052No Hit
TTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC930.134315424610052No Hit
CAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC920.13287117273252455No Hit
GCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC920.13287117273252455No Hit
CTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC920.13287117273252455No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC910.13142692085499713No Hit
TGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC910.13142692085499713No Hit
CTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC910.13142692085499713No Hit
AGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC910.13142692085499713No Hit
CTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC910.13142692085499713No Hit
TCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC900.12998266897746968No Hit
TGCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC900.12998266897746968No Hit
GACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC890.12853841709994224No Hit
TCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC890.12853841709994224No Hit
GTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC890.12853841709994224No Hit
ACGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC880.1270941652224148No Hit
GGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC880.1270941652224148No Hit
TCTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC880.1270941652224148No Hit
ATACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC880.1270941652224148No Hit
AAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC880.1270941652224148No Hit
TACGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC870.12564991334488734No Hit
GTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC870.12564991334488734No Hit
CTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC870.12564991334488734No Hit
AATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC870.12564991334488734No Hit
TTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC870.12564991334488734No Hit
TTCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC860.1242056614673599No Hit
ACACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC860.1242056614673599No Hit
AATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC860.1242056614673599No Hit
GTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC850.12276140958983246No Hit
GTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC850.12276140958983246No Hit
TAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC850.12276140958983246No Hit
AAGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC840.12131715771230502No Hit
TATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC840.12131715771230502No Hit
GCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC840.12131715771230502No Hit
TTAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC840.12131715771230502No Hit
ACTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC840.12131715771230502No Hit
TACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC840.12131715771230502No Hit
CTCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC840.12131715771230502No Hit
AAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC840.12131715771230502No Hit
CAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC840.12131715771230502No Hit
CTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC830.11987290583477758No Hit
ATGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC830.11987290583477758No Hit
TAGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC830.11987290583477758No Hit
TCAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC830.11987290583477758No Hit
AACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC830.11987290583477758No Hit
CTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC820.11842865395725014No Hit
TTGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC820.11842865395725014No Hit
ATTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC820.11842865395725014No Hit
ACCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC820.11842865395725014No Hit
CGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC820.11842865395725014No Hit
GCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC810.1169844020797227No Hit
CCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC810.1169844020797227No Hit
CCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC810.1169844020797227No Hit
GTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC800.11554015020219525No Hit
AGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC800.11554015020219525No Hit
TGCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC800.11554015020219525No Hit
TCGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC800.11554015020219525No Hit
ACTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC790.11409589832466782No Hit
CGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC790.11409589832466782No Hit
AGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC790.11409589832466782No Hit
ACGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC790.11409589832466782No Hit
CGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC780.11265164644714037No Hit
ACCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC780.11265164644714037No Hit
ATCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC780.11265164644714037No Hit
CCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC770.11120739456961294No Hit
TGTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC770.11120739456961294No Hit
TTTGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC770.11120739456961294No Hit
TCCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC770.11120739456961294No Hit
GTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC770.11120739456961294No Hit
TGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC770.11120739456961294No Hit
ACTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC770.11120739456961294No Hit
GGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC770.11120739456961294No Hit
CGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC760.1097631426920855No Hit
CCGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC760.1097631426920855No Hit
AACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC760.1097631426920855No Hit
ACAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC760.1097631426920855No Hit
GGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC760.1097631426920855No Hit
TAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC750.10831889081455806No Hit
ATTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC750.10831889081455806No Hit
TCGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC750.10831889081455806No Hit
GAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC750.10831889081455806No Hit
GGTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC750.10831889081455806No Hit
TTCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC740.10687463893703061No Hit
TAGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC740.10687463893703061No Hit
ACCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC740.10687463893703061No Hit
CGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC740.10687463893703061No Hit
TGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC740.10687463893703061No Hit
TTTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC730.10543038705950318No Hit
GCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC730.10543038705950318No Hit
GATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC730.10543038705950318No Hit
GCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC730.10543038705950318No Hit
GGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC730.10543038705950318No Hit
TCGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC730.10543038705950318No Hit
TGCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC730.10543038705950318No Hit
GGCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC720.10398613518197573No Hit
ATGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC720.10398613518197573No Hit
CTTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC720.10398613518197573No Hit
CATCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC720.10398613518197573No Hit
TGTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC720.10398613518197573No Hit
GTTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC710.1025418833044483No Hit
GAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC710.1025418833044483No Hit
ATAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC710.1025418833044483No Hit
GGACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC710.1025418833044483No Hit
CCCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC700.10109763142692085No Hit
GCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC700.10109763142692085No Hit
AGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC700.10109763142692085No Hit
CGGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC700.10109763142692085No Hit
GTGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC700.10109763142692085No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)