FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491917

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491917
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83669
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC950.11354265020497437No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC940.11234746441334306No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC940.11234746441334306No Hit
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC880.1051763496635552No Hit
TTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC850.10159079228866127No Hit
ATTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC850.10159079228866127No Hit
CTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC840.10039560649702996No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCT650.0295.01
TCGCCTA950.0295.02
GCCCCTA1350.0295.02
AGTCCTA1150.0295.02
AGCAAAT108.4510265E-4295.0295
AGTCCCT108.4510265E-4295.01
TGCCCCT400.0295.01
CGAACCT206.150913E-8295.01
TCCGCCT500.0295.01
GTACAGG108.4510265E-4295.0295
CTTCCTA1400.0295.02
CTTCCCT350.0295.01
CTTCCAC108.4510265E-4295.02
CGGCCTA800.0295.02
AGCCCTA1000.0295.02
GGGTCCT500.0295.01
TGGACCT255.275069E-10295.01
ATTACCT550.0295.01
GATCCCT400.0295.01
CAACCTA1150.0295.02
Produced by FastQC (version 0.11.5)