FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005491919

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491919
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130989
Sequences flagged as poor quality0
Sequence length301
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC2220.1694798799899228No Hit
CTATCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1930.1473406163876356No Hit
CTTACCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1920.14657719350479811No Hit
TCATCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1880.14352350197344815No Hit
ATCACCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1850.14123323332493568No Hit
TAATCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1840.1404698104420982No Hit
TCAACCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1820.13894296467642323No Hit
TTATCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1800.13741611891074823No Hit
CTAACCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1800.13741611891074823No Hit
ATCTCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1760.13436242737939827No Hit
TAAACCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1760.13436242737939827No Hit
TTCACCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1730.1320721587308858No Hit
CTCACCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1720.1313087358480483No Hit
TATTCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1720.1313087358480483No Hit
TTCTCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1720.1313087358480483No Hit
TTTTCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1710.1305453129652108No Hit
TACACCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1690.12901846719953586No Hit
AAAACCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1660.12672819855102335No Hit
CAATCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1650.12596477566818587No Hit
ATATCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1650.12596477566818587No Hit
TTGTCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1640.12520135278534839No Hit
TGCACCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1630.1244379299025109No Hit
ACAACCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1630.1244379299025109No Hit
TCGACCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1620.1236745070196734No Hit
AAATCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1590.12138423837116093No Hit
CACACCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1580.12062081548832346No Hit
ACCACCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1560.11909396972264846No Hit
ATAACCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1560.11909396972264846No Hit
TTACCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1560.11909396972264846No Hit
TTTACCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1560.11909396972264846No Hit
TCCACCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1540.11756712395697348No Hit
TCGTCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1540.11756712395697348No Hit
GGCACCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1540.11756712395697348No Hit
ACATCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1510.11527685530846102No Hit
TAGACCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1500.11451343242562352No Hit
CAGACCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1490.11375000954278604No Hit
TGGACCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1490.11375000954278604No Hit
TAGTCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1480.11298658665994854No Hit
TCTTCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1480.11298658665994854No Hit
TCACCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1470.11222316377711104No Hit
CAGTCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1470.11222316377711104No Hit
CCAACCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1470.11222316377711104No Hit
TATACCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1470.11222316377711104No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCC1460.11145974089427357No Hit
AACACCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1460.11145974089427357No Hit
GTAACCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1460.11145974089427357No Hit
ACTTCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1450.11069631801143608No Hit
GAATCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1440.10993289512859858No Hit
ACGACCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1430.1091694722457611No Hit
CACTCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1430.1091694722457611No Hit
GGGACCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1420.1084060493629236No Hit
CTCTCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1410.1076426264800861No Hit
TACTCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1400.10687920359724863No Hit
TACGCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1400.10687920359724863No Hit
TCCTCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1400.10687920359724863No Hit
CATTCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1400.10687920359724863No Hit
ATTACCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1390.10611578071441113No Hit
TAATCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCC1390.10611578071441113No Hit
ATGACCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1380.10535235783157365No Hit
GTTTCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1380.10535235783157365No Hit
TTGCCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1380.10535235783157365No Hit
TGAACCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1370.10458893494873615No Hit
GTACCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1370.10458893494873615No Hit
AACTCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1370.10458893494873615No Hit
TGATCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1370.10458893494873615No Hit
GTCACCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1370.10458893494873615No Hit
CAAACCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCC1370.10458893494873615No Hit
AATTCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1360.10382551206589866No Hit
CTTTCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1360.10382551206589866No Hit
AGAACCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1360.10382551206589866No Hit
CCCACCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1360.10382551206589866No Hit
GCAACCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1360.10382551206589866No Hit
TTTCCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1350.10306208918306116No Hit
CCTTCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1350.10306208918306116No Hit
AGCACCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1350.10306208918306116No Hit
AAGTCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1350.10306208918306116No Hit
GTATCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1340.10229866630022369No Hit
CTGTCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1340.10229866630022369No Hit
AGTACCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1340.10229866630022369No Hit
TAAACCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCC1340.10229866630022369No Hit
CATACCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1330.10153524341738619No Hit
TCAACCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCC1330.10153524341738619No Hit
ATGTCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1320.10077182053454871No Hit
GAGTCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1320.10077182053454871No Hit
ATTTCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1320.10077182053454871No Hit
ATATCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCC1320.10077182053454871No Hit
CGAACCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1310.10000839765171121No Hit
AATACCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1310.10000839765171121No Hit
GAGACCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1310.10000839765171121No Hit
TTCCCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1310.10000839765171121No Hit
TACCCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1310.10000839765171121No Hit
AAACCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1310.10000839765171121No Hit
TCTACCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1310.10000839765171121No Hit
TTCGCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCC1310.10000839765171121No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)