FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491920

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491920
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130989
Sequences flagged as poor quality0
Sequence length301
%GC54

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA48283.685805678339402No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA44473.3949415599783186No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA38232.918565681087725No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA38042.9040606463138126No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA37672.8758139996488254No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA36242.766644527403064No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA33302.5421981998488423No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA32232.460511951385231No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA31402.397147852109719No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA29692.2666025391445084No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA29652.2635488476131584No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA28532.1780454847353594No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA28452.1719381016726595No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA27382.090251853209048No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA26982.0597149378955484No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA26222.001694798799899No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA25431.9413843910557376No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA24261.8520639137637511No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA23401.786409545839727No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA22711.7337333669239403No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA21351.6299078548580415No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA20551.5688340242310423No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA20101.5344799945033551No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA19941.5222652283779554No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA19801.5115773080182306No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA18621.4214934078434067No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA16691.274152791455771No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA15001.1451343242562353No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA10540.8046477185107146No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA9200.702349052210491No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA8610.6573071021230791No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA7560.5771476994251425No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA6910.5275252120407057No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA6670.509203062852606No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA5660.4320973516860194No Hit
GGACTACAAGGGTATCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA5150.3931627846613074No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA5130.3916359388956325No Hit
GGACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA5090.3885822473642825No Hit
GGACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA4890.3733137897075327No Hit
GGACTACACGGGTATCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA4730.3610990235821328No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA4310.3290352625029583No Hit
GGACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA4080.311476536197696No Hit
GGACTACTCGGGTATCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA4070.3107131133148585No Hit
GGACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA4050.3091862675491835No Hit
GGACTACTAGGGTATCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA3900.29773492430662113No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA3880.2962080785409462No Hit
GGACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA3830.29239096412675875No Hit
GGACTACCCGGGTATCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA3810.2908641183610837No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA3760.2870470039468963No Hit
GGACTACAAGGGTTTCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA3580.2733053920558215No Hit
GGACTACACGGGTTTCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA3540.2702517005244715No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA3370.2572735115162342No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA3350.2557466657505592No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA3150.24047820809380943No Hit
GGACTACTCGGGTTTCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA3140.23971478521097192No Hit
GGACTACACGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA3100.23666109367962196No Hit
GGACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA3090.23589767079678445No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA3040.23208055638259703No Hit
GGACTACCGGGGTATCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA2860.2183389444915222No Hit
GGACTACTCGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA2780.21223156142882227No Hit
GGACTACTAGGGTTTCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA2770.21146813854598479No Hit
GGACTACCCGGGTTTCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA2760.2107047156631473No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA2750.2099412927803098No Hit
GGACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA2740.2091778698974723No Hit
GGACTACTAGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA2720.2076510241317973No Hit
GGACTACCCGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA2700.20612417836612232No Hit
GGACTACTGGGGTATCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA2670.2038339097176099No Hit
GGACTACCGGGGTTTCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA2550.19467283512355998No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA2320.17711410881829773No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA2310.17635068593546022No Hit
GGACTACCGGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA2290.17482384016978525No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA2220.1694798799899228No Hit
GGACTACTGGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA2180.16642618845857288No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA2170.16566276557573537No Hit
GGACTACTGGGGTTTCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA2170.16566276557573537No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA2050.15650169098168548No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA2040.155738268098848No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1730.1320721587308858No Hit
GGACTACACGGGTATCTAATCCTGTTCACTACCCATGCTTTCGAGCCTCA1710.1305453129652108No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1650.12596477566818587No Hit
GGACTACCCGGGTATCTAATCCTGTTCACTACCCATGCTTTCGAGCCTCA1620.1236745070196734No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1600.12214766125399842No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCACCTCA1580.12062081548832346No Hit
GGACTACTCGGGTATCTAATCCTGTTCACTACCCATGCTTTCGAGCCTCA1550.11833054683981098No Hit
GGACTACTAGGGTATCTAATCCTGTTCACTACCCATGCTTTCGAGCCTCA1500.11451343242562352No Hit
GGACTACAAGGGTATCTAATCCTGTTCACTACCCATGCTTTCGAGCCTCA1460.11145974089427357No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1420.1084060493629236No Hit
GGACTACAAGGGTTTCTAATCCTGTTCACTACCCATGCTTTCGAGCCTCA1410.1076426264800861No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1380.10535235783157365No Hit
GGACTACCAGGGTATCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA1370.10458893494873615No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCACCTCA1360.10382551206589866No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCACCTCA1340.10229866630022369No Hit
GGACTACACGGGTTTCTAATCCTGTTCACTACCCATGCTTTCGAGCCTCA1310.10000839765171121No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGTA8050.0295.08
CTACCGG12400.0295.04
TGGGTTT108.4564806E-4294.999979
ACTAGGC108.4564806E-4294.999976
ATTTTGC157.2257535E-6294.99997295
AGTTAGG305.456968E-12294.99997295
GGTCTAC206.164191E-8294.999971
CTAGGCT108.4564806E-4294.999977
GGCCTAC157.2257535E-6294.999971
GGACTAC123000.0294.640231
AGGGTAT24750.0294.404059
TACTCGG16550.0294.108765
ACTAGGG15750.0294.06356
CTAGGGT15700.0294.060527
CGGGTTT23250.0293.731179
GACTACT44650.0293.678622
TACACGG19300.0293.47155
ACGGGTT9350.0293.422458
ACTCGGG16600.0293.222876
CTCGGGT16450.0293.206677
Produced by FastQC (version 0.11.5)