FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491925

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491925
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67814
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC730.1076473884448639No Hit
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC710.10469814492582653No Hit
ATCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.10322352316630783No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC690.10174890140678915No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAA157.210412E-6295.0295
TTGTCCT500.0295.01
TGGTCCT255.2568794E-10295.01
AACGCCT500.0295.01
ACGCCTA1100.0295.02
GGGTCCT157.210412E-6295.01
ACGCCCT350.0295.01
CCTACGC157.210412E-6295.05
GTCACCT303.6379788E-12295.01
TCGACCT303.6379788E-12295.01
CTCCCTA1100.0295.02
CGCCCTA1200.0295.02
GGACCTA900.0295.02
GCGACCT157.210412E-6295.01
CGGGGGC303.6379788E-12295.09
AGGACCT157.210412E-6295.01
GTTCCTA1100.0295.02
GTCGCCT157.210412E-6295.01
GTACCGG157.210412E-6295.0295
GGCGCCT157.210412E-6295.01
Produced by FastQC (version 0.11.5)