FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491927

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491927
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58991
Sequences flagged as poor quality0
Sequence length301
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC730.1237476903256429No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC700.11866216880541099No Hit
ATATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC680.11527182112525639No Hit
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC670.1135766472851791No Hit
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.10849112576494718No Hit
AAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC640.10849112576494718No Hit
TTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC630.1067959519248699No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC610.1034056042447153No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC610.1034056042447153No Hit
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC610.1034056042447153No Hit
TCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC600.101710430404638No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC600.101710430404638No Hit
TTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC600.101710430404638No Hit
ATCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC590.1000152565645607No Hit
ATCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC590.1000152565645607No Hit
CTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC590.1000152565645607No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC590.1000152565645607No Hit
TATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC590.1000152565645607No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCCCT206.135633E-8295.01
ATCCTCG108.4447046E-4295.03
AGCCTAC2950.0295.03
AATCCTA1250.0295.02
AACGCCT350.0295.01
ACGCCTA900.0295.02
CCAACCT350.0295.01
GTCACCT450.0295.01
CCTACCT255.2568794E-10295.01
TATACCT255.2568794E-10295.01
TGACCTA650.0295.02
CTCCCTA950.0295.02
CGCCCTA750.0295.02
AAATCCT450.0295.01
TGCGCCT206.135633E-8295.01
TTACCCT206.135633E-8295.01
GATACCT350.0295.01
CGCCTAC2500.0295.03
ACGTCCT206.135633E-8295.01
GTTCCTA1050.0295.02
Produced by FastQC (version 0.11.5)