FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491931

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491931
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72241
Sequences flagged as poor quality0
Sequence length301
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCCT255.2568794E-10294.995181
GGCGCCT255.2568794E-10294.995181
CGATCCT255.2568794E-10294.995181
GCCCCTA1100.0294.995152
TGCCCCT206.1456376E-8294.995151
AGATCCT350.0294.995151
GCGACCT303.6379788E-12294.995151
CTTCCCT350.0294.995151
CCCTACT108.44905E-4294.995154
GTCCTAC4150.0294.995153
GGTCCTA700.0294.995152
GATCCCT157.2128223E-6294.995151
CGCGCCT303.6379788E-12294.995151
GCGCCTA900.0294.995152
GCGCCCT450.0294.995151
TACGGGG800.0294.995157
CTCGGAG108.44905E-4294.995156
CCCCCCT450.0294.995151
GAGCCTA600.0294.995152
CTCGCCT206.1456376E-8294.995151
Produced by FastQC (version 0.11.5)