FastQCFastQC Report
Wed 8 May 2024
EGAF00005491933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76681
Total Bases23 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATACCCTTGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1410.1838786661624131Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGAAACCCTTGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1350.17605404207039552Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGAAACCCTAGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1040.13562681759497136Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGATACCCTAGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1030.13432271357963513Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)