FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76681
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATACCCTTGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1410.1838786661624131Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGAAACCCTTGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1350.17605404207039552Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGAAACCCTAGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1040.13562681759497136Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGATACCCTAGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1030.13432271357963513Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGCCT206.1278115E-8295.190581
CGTGCCT108.43331E-4295.190581
GGGTCCT303.6379788E-12295.190581
CGGCCCT206.1278115E-8295.190581
CCGGCCT206.1278115E-8295.190581
TCCCTCG108.43331E-4295.190583
CGCGCCT206.1278115E-8295.190581
GCGCCCT303.6379788E-12295.190581
AGTTCCT350.0295.190581
GGGCCCT157.195489E-6295.190581
TAGGCCT400.0295.190581
TGGGCCT108.43331E-4295.190581
GTTGCCT108.43331E-4295.190581
AGGTCCT500.0295.190581
GTACCGG108.449818E-4294.99805295
ACGGGGC157.2142757E-6294.998058
CGGGGGG157.2142757E-6294.998059
CGGGGCA157.2142757E-6294.998059
GCCTACG13200.0292.76324
CCTACGG69700.0292.246985
Produced by FastQC (version 0.11.5)