FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491934

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491934
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76681
Sequences flagged as poor quality0
Sequence length301
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACAAGGGTATCTAATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA4680.6103206791773712Illumina Single End PCR Primer 1 (100% over 32bp)
GGACTACAAGGGTTTCTAATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA4210.5490277904565668Illumina Single End PCR Primer 1 (100% over 32bp)
GGACTACTAGGGTATCTAATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA3520.45904461339836466Illumina Single End PCR Primer 1 (100% over 32bp)
GGACTACTAGGGTTTCTAATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA3310.4316584290763031Illumina Single End PCR Primer 1 (100% over 32bp)
GGACTACTCGGGTATCTAATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA2480.3234177958033933Illumina Single End PCR Primer 1 (100% over 32bp)
GGACTACACGGGTATCTAATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA2350.30646444360402186Illumina Single End PCR Primer 1 (100% over 32bp)
GGACTACAAGGGTATCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA2210.28820698738931416No Hit
GGACTACAAGGGTATCTAATGTGGTCGCCGTATCATTGCTGCCTCCCGTA2100.2738618432206153No Hit
GGACTACACGGGTTTCTAATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA2040.2660372191285977Illumina Single End PCR Primer 1 (100% over 32bp)
GGACTACAAGGGTTTCTAATGTGGTCGCCGTATCATTGCTGCCTCCCGTA1980.2582125950365801No Hit
GGACTACTCGGGTTTCTAATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA1870.24386745086788122Illumina Single End PCR Primer 1 (100% over 32bp)
GGACTACAAGGGTTTCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA1870.24386745086788122No Hit
GGACTACTGGGGTATCTAATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA1810.23604282677586363Illumina Single End PCR Primer 1 (100% over 32bp)
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGAATCTCA1770.2308264107145186No Hit
GGACTACTAGGGTATCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA1700.22169768260716477No Hit
GGACTACAAGGGTTTCTCGGTGGTCGCCGTATCATTGCTGCCTCCCGTAG1690.22039357859182845Illumina RNA PCR Primer (95% over 23bp)
GGACTACAAGGGTATCTCGGTGGTCGCCGTATCATTGCTGCCTCCCGTAG1660.2164812665458197Illumina Single End PCR Primer 1 (95% over 24bp)
GGACTACAAGGGTTTCTCGTATGCCGTCTTCTGCTTGGCTGCCTCCCGTA1660.2164812665458197TruSeq Adapter, Index 27 (96% over 26bp)
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAATCTCA1620.21126485048447463No Hit
GGACTACTAGGGTATCTAATGTGGTCGCCGTATCATTGCTGCCTCCCGTA1610.20996074646913837No Hit
GGACTACTAGGGTTTCTCGTATGCCGTCTTCTGCTTGGCTGCCTCCCGTA1600.20865664245380214Illumina PCR Primer Index 6 (96% over 25bp)
GGACTACTGGGGTTTCTAATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA1570.20474433040779333Illumina Single End PCR Primer 1 (100% over 32bp)
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAATCTCA1460.19039918623909444No Hit
GGACTACTAGGGTTTCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA1430.18648687419308563No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1430.18648687419308563No Hit
GGACTACAAGGGTATCTAATGCCGTCTTCTGCTTGCCTGCTGCCTCCCGT1410.1838786661624131No Hit
GGACTACTAGGGTATCTCGGTGGTCGCCGTATCATTGCTGCCTCCCGTAG1370.17866225010106804Illumina Single End PCR Primer 1 (95% over 24bp)
GGACTACTAGGGTTTCTAATGTGGTCGCCGTATCATTGCTGCCTCCCGTA1330.173445834039723No Hit
GGACTACCCGGGTTTCTAATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA1250.16301300191703288Illumina Single End PCR Primer 1 (100% over 32bp)
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1210.15779658585568784No Hit
GGACTACCGGGGTTTCTAATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA1210.15779658585568784Illumina Single End PCR Primer 1 (100% over 32bp)
GGACTACCGGGGTATCTAATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA1190.15518837782501532Illumina Single End PCR Primer 1 (100% over 32bp)
GGACTACACGGGTATCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA1180.15388427380967906No Hit
GGACTACTCGGGTATCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA1150.14997196176367028No Hit
GGACTACTAGGGTATCTCGTATGCCGTCTTCTGCTTGGCTGCCTCCCGTA1120.14605964971766147Illumina PCR Primer Index 2 (96% over 27bp)
GGACTACTCGGGTATCTAATGTGGTCGCCGTATCATTGCTGCCTCCCGTA1100.14345144168698895No Hit
GGACTACAAGGGTTTCTAATGCCGTCTTCTGCTTGCCTGCTGCCTCCCGT1100.14345144168698895No Hit
GGACTACAAGGGTATCTAATCGGAAGAGCGTCGTGTAGGGACAGAGTGTA1100.14345144168698895Illumina Single End PCR Primer 1 (96% over 32bp)
GGACTACACGGGTTTCTCGTATGCCGTCTTCTGCTTGGCTGCCTCCCGTA1090.1421473376716527TruSeq Adapter, Index 27 (96% over 26bp)
GGACTACAAGGGTTTCTCGTATGCCGTCTTCTGCTTGCTGCCTCCCGTAG1080.14084323365631643TruSeq Adapter, Index 27 (96% over 26bp)
GGACTACAAGGGTATCTAATCGGAAGCGCGTCGTGTAGGGAAAGAGTGTA1070.13953912964098017Illumina Single End PCR Primer 1 (96% over 32bp)
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGAATCTCA1060.1382350256256439No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1060.1382350256256439No Hit
GGACTACTAGGGTATCTAATGCCGTCTTCTGCTTGCCTGCTGCCTCCCGT1040.13562681759497136No Hit
GGACTACAAGGGTATCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT1040.13562681759497136Illumina Single End PCR Primer 1 (100% over 33bp)
GGACTACAAGGGTATCTAAGTAGATCTCGGTGGTCGCCGTATCATTGCTG1030.13432271357963513Illumina Single End PCR Primer 1 (100% over 27bp)
GGACTACTAGGGTATCTAATGCCGTCTTCTGCTTGCTGCTGCCTCCCGTA1030.13432271357963513No Hit
GGACTACTAGGGTTTCTCGGTGGTCGCCGTATCATTGCTGCCTCCCGTAG1020.13301860956429884Illumina RNA PCR Primer (95% over 23bp)
GGACTACAAGGGTTTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT1020.13301860956429884Illumina Single End PCR Primer 1 (100% over 33bp)
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1010.1317145055489626No Hit
GGACTACCCGGGTATCTAATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA1010.1317145055489626Illumina Single End PCR Primer 1 (100% over 32bp)
GGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAATCTCA1000.13041040153362632No Hit
GGACTACTAGGGTTTCTCGTATGCCGTCTTCTGCTTGCTGCCTCCCGTAG1000.13041040153362632Illumina PCR Primer Index 6 (96% over 25bp)
GGACTACACGGGTTTCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA980.1278021935029538No Hit
GGACTACAAGGGTATCTAATGCCGTCTTCTGCTTGCTGCTGCCTCCCGTA970.12649808948761754No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA960.12519398547228128No Hit
GGACTACTGGGGTATCTAATGTGGTCGCCGTATCATTGCTGCCTCCCGTA960.12519398547228128No Hit
GGACTACAAGGGTATCTCGTATGCCGTCTTCTGCTTGGCTGCCTCCCGTA950.123889881456945Illumina PCR Primer Index 2 (96% over 27bp)
GGACTACACGGGTATCTAATGTGGTCGCCGTATCATTGCTGCCTCCCGTA940.12258577744160874No Hit
GGACTACTAGGGTTTCTAATGCCGTCTTCTGCTTGCCTGCTGCCTCCCGT930.12128167342627248No Hit
GGACTACAAGGGTTTCTCGTATGCCGTCTTCTGTTGGCTGCCTCCCGTAG890.11606525736492743TruSeq Adapter, Index 27 (95% over 22bp)
GGACTACTGGGGTTTCTCGTATGCCGTCTTCTGCTTGGCTGCCTCCCGTA880.11476115334959117Illumina PCR Primer Index 2 (96% over 26bp)
GGACTACTCGGGTTTCTCGTATGCCGTCTTCTGCTTGGCTGCCTCCCGTA880.11476115334959117Illumina PCR Primer Index 10 (96% over 25bp)
GGACTACTCGGGTTTCTAATGTGGTCGCCGTATCATTGCTGCCTCCCGTA870.1134570493342549No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA870.1134570493342549No Hit
GGACTACAAGGGTTTCTAATCGGAAGCGCGTCGTGTAGGGAAAGAGTGTA870.1134570493342549Illumina Single End PCR Primer 1 (96% over 32bp)
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA860.11215294531891863No Hit
GGACTACTGGGGTTTCTAATGTGGTCGCCGTATCATTGCTGCCTCCCGTA860.11215294531891863No Hit
GGACTACTAGGGTATCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT860.11215294531891863Illumina Single End PCR Primer 1 (100% over 33bp)
GGACTACAAGGGTTTCTAATCGGAAGAGCGTCGTGTAGGGACAGAGTGTA860.11215294531891863Illumina Single End PCR Primer 1 (96% over 32bp)
GGACTACTAGGGTATCTAATCGGAAGCGCGTCGTGTAGGGAAAGAGTGTA850.11084884130358238Illumina Single End PCR Primer 1 (96% over 32bp)
GGACTACTGGGGTATCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA840.10954473728824611No Hit
GGACTACAAGGGTATCTAATGCCGTCTTCTGCTTGAGAGTGTAGATCTCG830.10824063327290985No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA820.10693652925757359No Hit
GGACTACACGGGTTTCTAATGTGGTCGCCGTATCATTGCTGCCTCCCGTA820.10693652925757359No Hit
GGACTACAAGGGTATCTCGTATGCCGTCTTCTGCTTGCTGCCTCCCGTAG810.10563242524223732Illumina PCR Primer Index 2 (96% over 27bp)
GGACTACAAGGGTTTCTAATGCCGTCTTCTGCTTGCTGCTGCCTCCCGTA800.10432832122690107No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGAATCTCA800.10432832122690107No Hit
GGACTACACGGGTATCTAATGCCGTCTTCTGCTTGCCTGCTGCCTCCCGT800.10432832122690107No Hit
GGACTACTAGGGTATCTAATCGGAAGAGCGTCGTGTAGGGACAGAGTGTA800.10432832122690107Illumina Single End PCR Primer 1 (96% over 32bp)
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAATCTCA790.1030242172115648No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAATCTCA790.1030242172115648No Hit
GGACTACTAGGGTTTCTAATGCCGTCTTCTGCTTGCTGCTGCCTCCCGTA780.10172011319622853No Hit
GGACTACCCGGGTATCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA780.10172011319622853No Hit
GGACTACTGGGGTTTCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA780.10172011319622853No Hit
GGACTACTAGGGTTTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT770.10041600918089227Illumina Single End PCR Primer 1 (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTAGG108.450154E-4294.99414295
CTGGGGG108.450154E-4294.994147
GGACTAC69250.0293.88661
CTCGGGT7850.0291.236247
CTACCGG5450.0289.58144
CGGGTAT11700.0288.690869
ACTCGGG8100.0287.710336
GACTACT29600.0287.519622
ACCGGGG5500.0286.948826
TACTCGG8150.0285.945255
CTAGGGT14150.0285.61277
ACCCGGG5700.0284.643436
TACCGGG5600.0284.458625
CAAGGGT16350.0284.168647
TCGGGTT3750.0283.194348
GGGGTTT7500.0283.194349
TGGGGTT3650.0282.871068
CTGGGGT7300.0282.871067
TACCCGG5750.0282.168275
CCCGGGT5650.0281.941287
Produced by FastQC (version 0.11.5)