FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101558
Sequences flagged as poor quality0
Sequence length301
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1420.1398215797869198No Hit
TCCACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1320.12997498966108037No Hit
TTCACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1310.1289903306484964No Hit
TAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1210.119143740522657No Hit
TAATCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1190.1171744224974891No Hit
TCCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1180.11618976348490519No Hit
CATTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1180.11618976348490519No Hit
TTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1160.1142204454597373No Hit
CTATCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1160.1142204454597373No Hit
TTCTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1160.1142204454597373No Hit
TTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1160.1142204454597373No Hit
GTCACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1150.11323578644715335No Hit
TCAACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1140.1122511274345694No Hit
TACCCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1130.11126646842198545No Hit
TTAACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1130.11126646842198545No Hit
TTTTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1130.11126646842198545No Hit
TACTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1130.11126646842198545No Hit
TCCCCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1110.10929715039681759No Hit
CATACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1110.10929715039681759No Hit
TCACCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1110.10929715039681759No Hit
CCTTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1100.10831249138423364No Hit
TTCCCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1100.10831249138423364No Hit
TACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1100.10831249138423364No Hit
ATTCCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1090.1073278323716497No Hit
CACACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1090.1073278323716497No Hit
ATCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1080.10634317335906575No Hit
ATCACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1080.10634317335906575No Hit
CCATCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1080.10634317335906575No Hit
TAAACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1070.1053585143464818No Hit
CAATCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1060.10437385533389788No Hit
TTTACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1060.10437385533389788No Hit
ACATCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1060.10437385533389788No Hit
ATGTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1060.10437385533389788No Hit
CCACCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1060.10437385533389788No Hit
TTATCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1060.10437385533389788No Hit
TCTTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1050.10338919632131394No Hit
CTTTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1050.10338919632131394No Hit
TCTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1050.10338919632131394No Hit
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1040.10240453730872999No Hit
ATAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1040.10240453730872999No Hit
GTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1040.10240453730872999No Hit
CCCACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1040.10240453730872999No Hit
CTCACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1030.10141987829614604No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1030.10141987829614604No Hit
ACAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1030.10141987829614604No Hit
ACAACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1030.10141987829614604No Hit
TACACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1030.10141987829614604No Hit
AGCACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1020.1004352192835621No Hit
AAATCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1020.1004352192835621No Hit
CAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1020.1004352192835621No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCACCT550.0295.000031
ATGCCCT550.0295.000031
GCCCCTA1050.0295.02
GTCGCCT350.0295.01
AGATCCT500.0295.01
CGAACCT350.0295.01
ACGCCTA1500.0295.02
GCGACCT450.0295.01
TCACCCT450.0295.01
CTATCCT750.0295.01
CGGCCCT350.0295.01
TTGTCCT450.0295.01
GGTCCCT255.275069E-10295.01
AGGACCT350.0295.01
GGATCCT500.0295.01
GCGCCCT350.0295.01
CGTTCCT350.0295.01
GGCCCTA1150.0295.02
AGAACCT450.0295.01
GCACCTA2550.0295.02
Produced by FastQC (version 0.11.5)