FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491941

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491941
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72701
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCCT255.2568794E-10294.99521
GACCCCT255.2568794E-10294.99521
ATTCCCT255.2568794E-10294.99521
TGACCTA1000.0294.99522
ACCTAGG255.2568794E-10294.99524
TATTCCT500.0294.99521
CGGGCCT255.2568794E-10294.99521
GGGCCCT255.2568794E-10294.99521
AGCTCCT255.2568794E-10294.99521
GGACCCT255.2568794E-10294.99521
CATCCCT255.2568794E-10294.99521
GGTTCCT255.2568794E-10294.99521
CCTGCCT255.2568794E-10294.99521
GCCACCT255.2568794E-10294.99521
TGATCCT206.1458195E-8294.995181
GTTGATT108.4491674E-4294.99518295
GCCCCTA1150.0294.995182
GCCCCCT303.6379788E-12294.995181
GAGTGGG600.0294.99518295
AGTCCTA1200.0294.995182
Produced by FastQC (version 0.11.5)