FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68704
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1190.1732068001863065No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC1070.15574056823474616No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1070.15574056823474616No Hit
CCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1050.15282952957615278No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1030.1499184909175594No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1010.147007452258966No Hit
TCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1000.1455519329296693No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC990.14409641360037262No Hit
GTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC990.14409641360037262No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC980.14264089427107593No Hit
CTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC970.1411853749417792No Hit
TATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC950.13827433628318583No Hit
CAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC940.13681881695388914No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC930.13536329762459245No Hit
AACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC930.13536329762459245No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC920.13390777829529577No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC920.13390777829529577No Hit
CTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC920.13390777829529577No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC920.13390777829529577No Hit
TTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC910.13245225896599905No Hit
ATTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC910.13245225896599905No Hit
TGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC900.1309967396367024No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC890.12954122030740567No Hit
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC890.12954122030740567No Hit
ATCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC890.12954122030740567No Hit
TTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC880.12808570097810898No Hit
TGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC880.12808570097810898No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC880.12808570097810898No Hit
CTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC880.12808570097810898No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC880.12808570097810898No Hit
CGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC880.12808570097810898No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC870.12663018164881232No Hit
AAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC860.1251746623195156No Hit
CTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC860.1251746623195156No Hit
CTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC860.1251746623195156No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC850.12371914299021891No Hit
TATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC850.12371914299021891No Hit
CCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC850.12371914299021891No Hit
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC850.12371914299021891No Hit
TATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC840.12226362366092222No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC840.12226362366092222No Hit
TTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC830.12080810433162553No Hit
ATGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC830.12080810433162553No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC830.12080810433162553No Hit
CCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC830.12080810433162553No Hit
TCGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC820.11935258500232883No Hit
TGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC820.11935258500232883No Hit
AATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC820.11935258500232883No Hit
GTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC820.11935258500232883No Hit
GTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC810.11789706567303213No Hit
ACGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC810.11789706567303213No Hit
CTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC810.11789706567303213No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC810.11789706567303213No Hit
TCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC810.11789706567303213No Hit
ACTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC800.11644154634373545No Hit
CTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC800.11644154634373545No Hit
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC800.11644154634373545No Hit
CCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC800.11644154634373545No Hit
TAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC800.11644154634373545No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC790.11498602701443875No Hit
CAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC790.11498602701443875No Hit
TTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC790.11498602701443875No Hit
ACCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC790.11498602701443875No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC790.11498602701443875No Hit
GCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC790.11498602701443875No Hit
CCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC790.11498602701443875No Hit
TACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC790.11498602701443875No Hit
AAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC780.11353050768514204No Hit
TGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC780.11353050768514204No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC780.11353050768514204No Hit
CGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC780.11353050768514204No Hit
CACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC780.11353050768514204No Hit
CGTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC780.11353050768514204No Hit
TTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC780.11353050768514204No Hit
ACCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC780.11353050768514204No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC780.11353050768514204No Hit
CTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC770.11207498835584537No Hit
CATCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC770.11207498835584537No Hit
CTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC770.11207498835584537No Hit
GTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC770.11207498835584537No Hit
ACAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC770.11207498835584537No Hit
AGCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC760.11061946902654868No Hit
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC760.11061946902654868No Hit
AACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC760.11061946902654868No Hit
AACGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC760.11061946902654868No Hit
TCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC760.11061946902654868No Hit
ACGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC760.11061946902654868No Hit
ACACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC760.11061946902654868No Hit
ACAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC760.11061946902654868No Hit
ATTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC760.11061946902654868No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC760.11061946902654868No Hit
AAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC760.11061946902654868No Hit
TCAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC760.11061946902654868No Hit
AATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC760.11061946902654868No Hit
TCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC760.11061946902654868No Hit
TCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC760.11061946902654868No Hit
CATCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC750.10916394969725197No Hit
CAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC750.10916394969725197No Hit
ATCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC750.10916394969725197No Hit
TTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC750.10916394969725197No Hit
CTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC750.10916394969725197No Hit
ACTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC750.10916394969725197No Hit
GCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC750.10916394969725197No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC750.10916394969725197No Hit
TGCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC740.1077084303679553No Hit
TAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC740.1077084303679553No Hit
GTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC740.1077084303679553No Hit
CAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC740.1077084303679553No Hit
TTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC740.1077084303679553No Hit
TAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC740.1077084303679553No Hit
CATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC740.1077084303679553No Hit
TACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC740.1077084303679553No Hit
TTCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC740.1077084303679553No Hit
CCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC730.1062529110386586No Hit
CGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC730.1062529110386586No Hit
AGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC730.1062529110386586No Hit
CAGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC730.1062529110386586No Hit
AGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC730.1062529110386586No Hit
GACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC720.10479739170936189No Hit
TCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC720.10479739170936189No Hit
TGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC720.10479739170936189No Hit
TAGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC720.10479739170936189No Hit
CCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC720.10479739170936189No Hit
ATGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC720.10479739170936189No Hit
CGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC720.10479739170936189No Hit
TGTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC720.10479739170936189No Hit
ACCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC720.10479739170936189No Hit
TCGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC710.10334187238006522No Hit
GAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC710.10334187238006522No Hit
CGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC710.10334187238006522No Hit
CTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC710.10334187238006522No Hit
CTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC710.10334187238006522No Hit
GTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC710.10334187238006522No Hit
ACTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC700.10188635305076851No Hit
TTGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC700.10188635305076851No Hit
ACGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC700.10188635305076851No Hit
ATCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC700.10188635305076851No Hit
ACCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC700.10188635305076851No Hit
TAGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC700.10188635305076851No Hit
GCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC690.10043083372147181No Hit
GTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC690.10043083372147181No Hit
CCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC690.10043083372147181No Hit
TTAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC690.10043083372147181No Hit
ATGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC690.10043083372147181No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC690.10043083372147181No Hit
GGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC690.10043083372147181No Hit
TCGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC690.10043083372147181No Hit
TGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC690.10043083372147181No Hit
TGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC690.10043083372147181No Hit
TCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC690.10043083372147181No Hit
AAGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC690.10043083372147181No Hit
ACGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC690.10043083372147181No Hit
CGCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC690.10043083372147181No Hit
TCCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC690.10043083372147181No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCCTA700.0295.000032
GTGTCGG700.0295.00003295
CGTCCTA700.0295.000032
ATACCTA1400.0295.000032
GCATCCT350.0295.000031
TGTCCTA1400.0295.000032
TGATCCT650.0295.01
GCCCCTA900.0295.02
TCGCCCT157.2108287E-6295.01
ACAACCT303.6379788E-12295.01
AGTCCTA850.0295.02
AGATCCT206.142909E-8295.01
ACGCCTA950.0295.02
CAGCCTA950.0295.02
GAGTCCT157.2108287E-6295.01
TAGACCT206.142909E-8295.01
GATCCTA1350.0295.02
ATTACCT450.0295.01
TTGTCCT550.0295.01
TCCCTCG108.4477436E-4295.03
Produced by FastQC (version 0.11.5)