FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491946

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491946
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65227
Sequences flagged as poor quality0
Sequence length301
%GC51

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA14422.210740950833244No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA14232.1816119091787143No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA11871.819798549680347No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA11711.795268830392322No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA11641.7845370782038115No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA11431.7523418216382787No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA11431.7523418216382787No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA11401.747742499271774No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA11351.7400769619942662No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA10021.5361736704125593No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA9891.516243273491039No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA9791.5009121989360235No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA9631.4763824796479985No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA9561.4656507274594877No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA9391.439587900715961No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA9311.4273230410719489No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA9181.4073926441504285No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA9101.395127784506416No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA8761.3430021310193632No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA8631.3230717340978428No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA8321.2755454029772948No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA8161.2510156836892696No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA7751.1881582780137059No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA7721.1835589556472013No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA7641.1712940960031888No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA7601.1651616661811826No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA7481.146764376715164No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA7451.1421650543486592No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA7431.1390988394376562No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA7221.1069035828721234No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA7161.0977049381391142No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA7021.0762414337620925No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA6951.0655096815735816No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA6931.0624434666625784No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA6881.0547779293850705No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA6791.0409799622855567No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA6721.0302482100970458No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA6490.9949867386205099No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA6420.9842549864319989No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA6320.9689239118769835No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA6040.9259969031229399No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA6010.9213975807564352No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5760.8830698943688964No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA5750.8815367869133948No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5680.8708050347248839No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA5280.8094807365048217No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5280.8094807365048217No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4880.7481564382847593No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4770.7312922562742422No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4760.7297591488187407No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA4650.7128949668082236No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4560.6990969997087095No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3980.6101767672896193No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3840.5887132629125975No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1970.30202216873380655No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1930.2958897389118003No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1800.2759593419902801No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1770.27136001962377543No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA1730.26522758980176925No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1720.26369448234626763No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1710.2621613748907661No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA1620.24836340779125207No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1610.24683030033575054No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1540.23609854814723966No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1510.23149922578073498No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1490.22843301086973186No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1470.22536679595872877No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1320.20237018412620542No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1300.1993039692152023No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA1280.1962377543041992No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1280.1962377543041992No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA1270.19470464684869762No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA1260.19317153939319606No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1220.18703910957118983No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1190.18243978720468518No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1170.17937357229368206No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA1170.17937357229368206No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1160.1778404648381805No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1160.1778404648381805No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1150.17630735738267894No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA1150.17630735738267894No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1150.17630735738267894No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1140.17477424992717738No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1130.17324114247167585No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1110.17017492756067273No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1110.17017492756067273No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA1100.16864182010517118No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1080.16557560519416806No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1060.16250939028316494No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1050.1609762828276634No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1040.15944317537216185No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1030.15791006791666026No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1020.1563769604611587No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1010.15484385300565717No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA1010.15484385300565717No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA960.14717831572814938No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA950.14564520827264782No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA930.14257899336164473No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA930.14257899336164473No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA910.1395127784506416No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA910.1395127784506416No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA900.13797967099514005No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA890.1364465635396385No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA890.1364465635396385No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA890.1364465635396385No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA880.13491345608413693No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA880.13491345608413693No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA880.13491345608413693No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA870.13338034862863538No Hit
GGACTACAAGGGTTTCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA870.13338034862863538No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA860.13184724117313382No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA850.13031413371763229No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA850.13031413371763229No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA840.12878102626213073No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA820.12571481135112758No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA810.12418170389562604No Hit
GGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA810.12418170389562604No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA790.12111548898462293No Hit
GGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA770.11804927407361983No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA760.11651616661811826No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA750.1149830591626167No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA740.11344995170711515No Hit
GGACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA740.11344995170711515No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA740.11344995170711515No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA720.11038373679611205No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA720.11038373679611205No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA710.10885062934061049No Hit
GGACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA710.10885062934061049No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA710.10885062934061049No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA700.10731752188510894No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA700.10731752188510894No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA700.10731752188510894No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA690.10578441442960736No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA680.10425130697410581No Hit
GGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA660.10118509206310271No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTCTC108.446757E-4295.09
GTTTGCC750.0295.0295
CGTTAGC700.0295.0295
ATTAGTC157.2091443E-6295.0295
ATTCGCC255.2568794E-10295.0295
GGTAGCC206.1405444E-8295.0295
GGCTACT108.446757E-4295.01
AGTTAGT108.446757E-4295.0295
AGTTAGG108.446757E-4295.0295
GTTCGCC108.446757E-4295.0295
GGACTAC60200.0294.754971
ACGGGTA3350.0290.597028
ACACGGG7400.0289.020266
TACACGG7400.0289.020265
CTACCGG5000.0286.150024
GGGGTTT8600.0284.70939
TCGGGTA4050.0284.074078
CGGGTAT10450.0283.708139
TGGGGTA2600.0283.653848
ATTTGCC2600.0283.65384295
Produced by FastQC (version 0.11.5)