FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491947

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491947
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91482
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1600.1748977941015719No Hit
ATTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1540.16833912682276295No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1520.1661529043964933No Hit
TAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1470.16068734833081919No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1440.15740801469141472No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1440.15740801469141472No Hit
CTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1430.1563149034782799No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1420.15522179226514504No Hit
CCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1420.15522179226514504No Hit
ATCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1410.15412868105201022No Hit
CCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1380.15084934741260575No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1380.15084934741260575No Hit
TACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1360.1486631249863361No Hit
TCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1360.1486631249863361No Hit
CTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1350.14757001377320128No Hit
TTCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1340.14647690256006646No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1330.14538379134693163No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1330.14538379134693163No Hit
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1320.1442906801337968No Hit
ACACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1290.14101134649439234No Hit
GTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1280.13991823528125752No Hit
CATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1270.1388251240681227No Hit
CAGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1260.13773201285498787No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1250.13663890164185302No Hit
TTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1240.13554579042871823No Hit
TTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1240.13554579042871823No Hit
ATCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1240.13554579042871823No Hit
CAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1230.1344526792155834No Hit
GCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1230.1344526792155834No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1220.13335956800244855No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1220.13335956800244855No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1220.13335956800244855No Hit
CCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1220.13335956800244855No Hit
ACTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1210.13226645678931376No Hit
TATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1210.13226645678931376No Hit
AAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1200.13117334557617893No Hit
CACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1200.13117334557617893No Hit
CCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1200.13117334557617893No Hit
TATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1190.13008023436304408No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1190.13008023436304408No Hit
TTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1190.13008023436304408No Hit
ATCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1180.12898712314990926No Hit
GTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1180.12898712314990926No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1180.12898712314990926No Hit
TAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1180.12898712314990926No Hit
ACTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1170.12789401193677447No Hit
ACAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1170.12789401193677447No Hit
TTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1160.12680090072363961No Hit
CTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1160.12680090072363961No Hit
GTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1130.12352156708423515No Hit
TTGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1130.12352156708423515No Hit
CTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1130.12352156708423515No Hit
TTCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1130.12352156708423515No Hit
TCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1130.12352156708423515No Hit
CAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1130.12352156708423515No Hit
GTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1130.12352156708423515No Hit
TAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1120.12242845587110032No Hit
ATGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1120.12242845587110032No Hit
GTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1120.12242845587110032No Hit
CACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1110.12133534465796551No Hit
TGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1110.12133534465796551No Hit
AGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1100.12024223344483068No Hit
TCCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1100.12024223344483068No Hit
CAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1100.12024223344483068No Hit
AACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1100.12024223344483068No Hit
TGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1100.12024223344483068No Hit
ATTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1100.12024223344483068No Hit
CATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1090.11914912223169585No Hit
TCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1090.11914912223169585No Hit
ATACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1090.11914912223169585No Hit
CCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1090.11914912223169585No Hit
TGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1080.11805601101856102No Hit
TTGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1080.11805601101856102No Hit
ACGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1080.11805601101856102No Hit
AAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1070.11696289980542621No Hit
TGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1070.11696289980542621No Hit
AATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1070.11696289980542621No Hit
CTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1060.11586978859229138No Hit
CCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1060.11586978859229138No Hit
AACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1060.11586978859229138No Hit
TCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1060.11586978859229138No Hit
TGTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1060.11586978859229138No Hit
CGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1050.11477667737915655No Hit
AGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1050.11477667737915655No Hit
AAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1040.11368356616602174No Hit
GTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1040.11368356616602174No Hit
ATTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1030.11259045495288691No Hit
CTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1030.11259045495288691No Hit
TGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1030.11259045495288691No Hit
ACCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1030.11259045495288691No Hit
AGCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1020.11149734373975208No Hit
CGACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1020.11149734373975208No Hit
GTGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1010.11040423252661727No Hit
CACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1010.11040423252661727No Hit
CGCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1010.11040423252661727No Hit
AAGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1010.11040423252661727No Hit
GCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1000.10931112131348243No Hit
ACCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1000.10931112131348243No Hit
GGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1000.10931112131348243No Hit
TCAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1000.10931112131348243No Hit
TGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC990.10821801010034761No Hit
CAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC990.10821801010034761No Hit
CTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC990.10821801010034761No Hit
CCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC990.10821801010034761No Hit
GTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC980.10712489888721277No Hit
TAGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC980.10712489888721277No Hit
ATGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC970.10603178767407796No Hit
TATCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC970.10603178767407796No Hit
GACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC970.10603178767407796No Hit
AGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC970.10603178767407796No Hit
AGGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC970.10603178767407796No Hit
GTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC970.10603178767407796No Hit
AATCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC960.10493867646094314No Hit
GACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC960.10493867646094314No Hit
CCAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC960.10493867646094314No Hit
GGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC960.10493867646094314No Hit
TAGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC960.10493867646094314No Hit
CGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC960.10493867646094314No Hit
TGCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC960.10493867646094314No Hit
TACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC950.1038455652478083No Hit
AGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC950.1038455652478083No Hit
TCTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC950.1038455652478083No Hit
TCGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC950.1038455652478083No Hit
CGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC950.1038455652478083No Hit
CAAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC940.1027524540346735No Hit
CTAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC940.1027524540346735No Hit
AGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC940.1027524540346735No Hit
AGTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC940.1027524540346735No Hit
ATGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC940.1027524540346735No Hit
TTTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC940.1027524540346735No Hit
CTTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC940.1027524540346735No Hit
ATGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC940.1027524540346735No Hit
ACCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC930.10165934282153867No Hit
CGGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC930.10165934282153867No Hit
TCCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC920.10056623160840383No Hit
GGTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC920.10056623160840383No Hit
CAGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC920.10056623160840383No Hit
GAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC920.10056623160840383No Hit
GCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC920.10056623160840383No Hit
GGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC920.10056623160840383No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCTAC3700.0295.03
TTGTCCT350.0295.01
ACGCCTA1450.0295.02
TGCACCT650.0295.01
GGGTCCT350.0295.01
GTCACCT550.0295.01
CCTACCT255.275069E-10295.01
CACACCT350.0295.01
CTCCCTA1300.0295.02
TTACCCT850.0295.01
CTCCCCT450.0295.01
GGACCTA1100.0295.02
AATTCCT350.0295.01
CACGCCT450.0295.01
GGTCCCT255.275069E-10295.01
TAGCCTA1100.0295.02
TGATCCT350.0295.01
TAGCCCT350.0295.01
GAGCCTA950.0295.02
TTTTCCT450.0295.01
Produced by FastQC (version 0.11.5)