FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491949

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491949
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82933
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACCT400.0295.000031
GGATCCT350.0295.000031
CCTACGA108.45089E-4295.000035
CGCCTAG108.45089E-4295.000033
TCGGAGG108.45089E-4295.00003295
GTGACCT400.0295.000031
CGATCCT206.150549E-8295.000031
TAGTCCT206.150549E-8295.000031
TCGCCCT255.275069E-10295.01
GATCCTA1050.0295.02
ACCTCCT255.275069E-10295.01
AAGCCTA550.0295.02
GTAGCCT550.0295.01
TACGCCT303.6379788E-12295.01
GGGCCTA1050.0295.02
CTCGCCT500.0295.01
CGTACCT255.275069E-10295.01
GAACCTA1650.0295.02
TAGGCCT450.0295.01
GACGCCT255.275069E-10295.01
Produced by FastQC (version 0.11.5)