FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491951

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491951
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80035
Sequences flagged as poor quality0
Sequence length301
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC1270.15868057724745424No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC1260.15743112388330105No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC1260.15743112388330105No Hit
CTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC1200.14993440369838196No Hit
CTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC1190.14868495033422877No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC1190.14868495033422877No Hit
TTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC1170.1461860436059224No Hit
CAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC1170.1461860436059224No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC1160.14493659024176922No Hit
CCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC1160.14493659024176922No Hit
CTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC1160.14493659024176922No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1160.14493659024176922No Hit
TTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC1150.14368713687761603No Hit
TACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC1150.14368713687761603No Hit
TAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC1130.14118823014930967No Hit
TCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC1120.13993877678515648No Hit
GCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC1110.1386893234210033No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC1110.1386893234210033No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC1110.1386893234210033No Hit
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC1110.1386893234210033No Hit
ACTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC1100.13743987005685013No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC1100.13743987005685013No Hit
ATTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC1100.13743987005685013No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC1090.13619041669269694No Hit
TTTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC1080.13494096332854374No Hit
TATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC1070.13369150996439058No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1070.13369150996439058No Hit
TACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1070.13369150996439058No Hit
GTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC1060.1324420566002374No Hit
CACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC1060.1324420566002374No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC1060.1324420566002374No Hit
TTCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC1060.1324420566002374No Hit
ATCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC1050.13119260323608423No Hit
CCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC1050.13119260323608423No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC1040.12994314987193104No Hit
AACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC1040.12994314987193104No Hit
CCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC1040.12994314987193104No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1030.12869369650777784No Hit
CTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC1030.12869369650777784No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1020.12744424314362468No Hit
TATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1010.1261947897794715No Hit
GTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC1010.1261947897794715No Hit
TTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC1000.12494533641531831No Hit
TCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC1000.12494533641531831No Hit
CCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC990.12369588305116512No Hit
ATCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC990.12369588305116512No Hit
ACGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC990.12369588305116512No Hit
CTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC990.12369588305116512No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC990.12369588305116512No Hit
ATTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC990.12369588305116512No Hit
ACCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC980.12244642968701194No Hit
TAGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC980.12244642968701194No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC980.12244642968701194No Hit
TGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC980.12244642968701194No Hit
TTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC980.12244642968701194No Hit
TCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC970.12119697632285875No Hit
AAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC970.12119697632285875No Hit
AACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC970.12119697632285875No Hit
CCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC970.12119697632285875No Hit
GTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC970.12119697632285875No Hit
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC970.12119697632285875No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC960.11994752295870557No Hit
ACCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC960.11994752295870557No Hit
CCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC960.11994752295870557No Hit
CAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC960.11994752295870557No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC950.11869806959455238No Hit
CATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC950.11869806959455238No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC950.11869806959455238No Hit
TACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC950.11869806959455238No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC940.1174486162303992No Hit
GGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC940.1174486162303992No Hit
ATACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC940.1174486162303992No Hit
TTAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC940.1174486162303992No Hit
ATGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC940.1174486162303992No Hit
TCAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC940.1174486162303992No Hit
GGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC940.1174486162303992No Hit
CCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC930.11619916286624603No Hit
TCTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC930.11619916286624603No Hit
ACACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC930.11619916286624603No Hit
AAGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC930.11619916286624603No Hit
ATCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC920.11494970950209284No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC920.11494970950209284No Hit
GACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC920.11494970950209284No Hit
AACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC920.11494970950209284No Hit
TGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC910.11370025613793966No Hit
CACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC910.11370025613793966No Hit
AAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC910.11370025613793966No Hit
TTCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC910.11370025613793966No Hit
AAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC910.11370025613793966No Hit
TAAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC900.11245080277378647No Hit
GCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC900.11245080277378647No Hit
TCCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC900.11245080277378647No Hit
ATGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC900.11245080277378647No Hit
GTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC900.11245080277378647No Hit
ATACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC900.11245080277378647No Hit
CTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC900.11245080277378647No Hit
ATTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC890.11120134940963329No Hit
CTTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC890.11120134940963329No Hit
ACAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC890.11120134940963329No Hit
TCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC890.11120134940963329No Hit
CATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC890.11120134940963329No Hit
TCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC880.1099518960454801No Hit
CAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC880.1099518960454801No Hit
TTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC880.1099518960454801No Hit
CGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC880.1099518960454801No Hit
TCCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC880.1099518960454801No Hit
ATCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC880.1099518960454801No Hit
TATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC880.1099518960454801No Hit
ACTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC870.10870244268132692No Hit
CTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC870.10870244268132692No Hit
TACGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC870.10870244268132692No Hit
GTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC870.10870244268132692No Hit
GTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC870.10870244268132692No Hit
TAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC860.10745298931717373No Hit
CTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC860.10745298931717373No Hit
CACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC860.10745298931717373No Hit
CATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC860.10745298931717373No Hit
ATCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC860.10745298931717373No Hit
AAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC860.10745298931717373No Hit
ACTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC860.10745298931717373No Hit
TGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC850.10620353595302055No Hit
GACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC850.10620353595302055No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC850.10620353595302055No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC850.10620353595302055No Hit
GCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC850.10620353595302055No Hit
TGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC850.10620353595302055No Hit
TGTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC850.10620353595302055No Hit
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC840.10495408258886738No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC840.10495408258886738No Hit
GACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC840.10495408258886738No Hit
AGCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC840.10495408258886738No Hit
CACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC840.10495408258886738No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC840.10495408258886738No Hit
ACGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC840.10495408258886738No Hit
CTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC840.10495408258886738No Hit
CCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC840.10495408258886738No Hit
TTTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC830.10370462922471418No Hit
TTGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC830.10370462922471418No Hit
AACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC830.10370462922471418No Hit
TTGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC830.10370462922471418No Hit
TCTGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC830.10370462922471418No Hit
TCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC830.10370462922471418No Hit
CGTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC830.10370462922471418No Hit
CCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC820.102455175860561No Hit
GAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC820.102455175860561No Hit
TACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC820.102455175860561No Hit
TCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC820.102455175860561No Hit
TAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC820.102455175860561No Hit
CGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC820.102455175860561No Hit
GCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC820.102455175860561No Hit
AAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC810.10120572249640782No Hit
ACGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC810.10120572249640782No Hit
ATTGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC810.10120572249640782No Hit
GTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC810.10120572249640782No Hit
CGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC810.10120572249640782No Hit
TTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC810.10120572249640782No Hit
AAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC810.10120572249640782No Hit
TGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC810.10120572249640782No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCCTA1450.0295.000032
CCACCTA1450.0295.000032
AGTCCTA950.0295.000032
CTACCTA1450.0295.000032
CGTTCCT350.0295.01
ATCCCCT206.1492756E-8295.01
AGCCCCT206.1492756E-8295.01
TTGTCCT500.0295.01
TGGTCCT255.275069E-10295.01
TCCTCCT500.0295.01
ACGCCTA800.0295.02
TTCACCT400.0295.01
TGCACCT108.4503385E-4295.01
CCAACCT255.275069E-10295.01
GGGTCCT450.0295.01
GCCTCCT206.1492756E-8295.01
CCTACCT400.0295.01
GGCACCT206.1492756E-8295.01
TAGGAGG108.4503385E-4295.07
ATCTCCT500.0295.01
Produced by FastQC (version 0.11.5)