FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491952

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491952
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80035
Sequences flagged as poor quality0
Sequence length301
%GC50

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA30033.752108452552008No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA29893.7346161054538642No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA29173.6446554632348347No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA29013.624664209408384No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA23042.8787405510089337No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA22782.8462547635409505No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA20902.6113575310801522No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA20402.548884862872493No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA19842.478915474479915No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA19572.445180233647779No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA18782.3464734178796776No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA18312.287749109764478No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA18262.281501842943712No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA18172.2702567626663335No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA17132.1403136127944022No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA16542.0665958643093645No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA15721.9641406884488035No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA15581.946648341350659No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA7200.8996064221902917No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA6610.8258886737052539No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA6560.819641406884488No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA6240.7796588992315862No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA5390.6734553632785656No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA5190.648466295995502No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA4630.5784969076029237No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA4590.5734990941463111No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA4480.5597551071406259No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA4320.539763853314175No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA4130.5160242393952645No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA4070.5085275192103454No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA4000.49978134566127325No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA3870.48353845192728184No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA3740.4672955581932904No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATGA3690.46104829137252457No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA3640.45480102455175864No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA3530.4410570375460736No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGA3470.43356031736115447No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA3450.43106141063284814No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGA3450.43106141063284814No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3420.42731305054038854No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATGA3390.423564690447929No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3360.4198163303554695No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA3330.41606797026300996No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3070.3835821827950272No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA2930.36608983569688264No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2840.354844755419504No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATGA2770.3460985818704317No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA2750.3435996751421253No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGA2720.33985131504966576No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA2710.3386018616855126No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2530.31611170113075526No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2480.3098644343099894No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA2430.30361716748922346No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATGA2400.2998688073967639No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGA2380.29736990066845753No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGA2360.29487099394015115No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2350.293621540575998No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATGA2340.2923720872118448No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATGA2330.29112263384769166No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2310.2886237271193853No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA2210.2761291934778534No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATGA2160.2698819266570875No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2130.266133566564628No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGA2110.2636346598363216No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA2100.26238520647216845No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA2090.26113575310801523No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATGA2050.2561379396514025No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2040.25488848628724936No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2040.25488848628724936No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGA2020.252389579558943No Hit
GGACTACACGGGTTTCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA1990.2486412194664834No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1980.24739176610233024No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1960.24489285937402389No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA1940.2423939526457175No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1940.2423939526457175No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA1940.2423939526457175No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1910.23864559255325793No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA1880.2348972324607984No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA1870.2336477790966452No Hit
GGACTACTCGGGTTTCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA1860.23239832573249206No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA1860.23239832573249206No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATGA1810.2261510589117261No Hit
GGACTACACGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA1750.218654338726807No Hit
GGACTACTCGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA1740.21740488536265384No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA1730.21615543199850062No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1730.21615543199850062No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGA1720.21490597863434746No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1710.21365652527019427No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1680.20990816517773475No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGA1660.20740925844942837No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1660.20740925844942837No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1640.204910351721122No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA1630.20366089835696885No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA1600.19991253826450928No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1550.19366527144374335No Hit
GGACTACCCGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA1540.19241581807959018No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA1530.191166364715437No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1520.1899169113512838No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1440.17992128443805835No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA1440.17992128443805835No Hit
GGACTACCCGGGTTTCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA1420.177422377709752No Hit
GGACTACAAGGGTTTCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA1390.17367401761729245No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1380.17242456425313926No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1340.16742675079652652No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1320.16492784406822017No Hit
GGACTACTAGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA1290.1611794839757606No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1270.15868057724745424No Hit
GGACTACAAGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA1260.15743112388330105No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA1240.1549322171549947No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1230.1536827637908415No Hit
GGACTACAGGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA1230.1536827637908415No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA1220.1524333104266883No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1210.15118385706253515No Hit
GGACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1200.14993440369838196No Hit
GGACTACTAGGGTTTCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA1190.14868495033422877No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1190.14868495033422877No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1190.14868495033422877No Hit
GGACTACAGGGGTTTCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA1170.1461860436059224No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1160.14493659024176922No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1160.14493659024176922No Hit
GGACTACTGGGGTTTCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA1150.14368713687761603No Hit
GGACTACTGGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA1150.14368713687761603No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1130.14118823014930967No Hit
GGACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA1110.1386893234210033No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1090.13619041669269694No Hit
GGACTACCGGGGTTTCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA1090.13619041669269694No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1080.13494096332854374No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1080.13494096332854374No Hit
GGACTACCAGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA1080.13494096332854374No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1070.13369150996439058No Hit
GGACTACCGGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA1070.13369150996439058No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1070.13369150996439058No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA1050.13119260323608423No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1040.12994314987193104No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1040.12994314987193104No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1020.12744424314362468No Hit
GGACTACCAGGGTTTCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA1010.1261947897794715No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1000.12494533641531831No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1000.12494533641531831No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1000.12494533641531831No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA970.12119697632285875No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA960.11994752295870557No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA950.11869806959455238No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA940.1174486162303992No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA910.11370025613793966No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA890.11120134940963329No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA890.11120134940963329No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA880.1099518960454801No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA880.1099518960454801No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA860.10745298931717373No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA830.10370462922471418No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGGT108.4503385E-4295.06
ACCGGGG6350.0295.06
CGGGGTA3600.0295.08
AGTAGCC108.4503385E-4295.0295
GGACTAC76150.0295.01
AGCTAGC108.4503385E-4295.0295
TGGGGTA2950.0295.08
CAGGGTA3300.0295.08
TACCGGG6450.0295.05
GAACTAC255.275069E-10295.01
AACTACC108.4503385E-4295.02
TACTGGG6400.0295.05
GTTAGGC108.4503385E-4295.0295
GGCTACC206.1492756E-8295.01
ACTGGGG6400.0295.06
TACCAGG6900.0295.05
TTAGCCC206.1492756E-8295.0295
CTACCGG6450.0295.04
ACTACTA8400.0295.03
ACTACCG6450.0295.03
Produced by FastQC (version 0.11.5)