FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491953

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491953
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70884
Sequences flagged as poor quality0
Sequence length301
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCT108.448302E-4295.01
GTTGAGA157.21178E-6295.0295
AGTCCTA1100.0295.02
TTGGCCT157.21178E-6295.01
ATCTCCT350.0295.01
GTCGCCT206.144364E-8295.01
AGTCCCT206.144364E-8295.01
AGTCCAC108.448302E-4295.02
AGATCCT108.448302E-4295.01
TCCGCCT255.2568794E-10295.01
TCACCCT255.2568794E-10295.01
GTTTCCT550.0295.01
CAGCCTA400.0295.02
CGGCCTA700.0295.02
TTAGCCT400.0295.01
GAGTCCT303.6379788E-12295.01
ACACCTA1500.0295.02
ACCGCCT350.0295.01
ACACCCT255.2568794E-10295.01
GCAACCT350.0295.01
Produced by FastQC (version 0.11.5)