FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491955

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491955
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89789
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC910.10134871754892023No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCCTA850.0295.000032
TCGCCTA1250.0295.02
CGGGTGG108.452053E-4295.09
GTCGCCT157.2181883E-6295.01
TGCCCCT303.6379788E-12295.01
AGATCCT400.0295.01
GAGTCCT450.0295.01
GATCCTA1750.0295.02
CTCCCTA1350.0295.02
TTGTCCT500.0295.01
GTCTCGT255.275069E-10295.0295
CGCGCCT450.0295.01
GGATCCT650.0295.01
GCGCCCT400.0295.01
TCCTATG108.452053E-4295.04
CGTTCCT350.0295.01
TAGCCCT350.0295.01
ACGGGAC157.2181883E-6295.08
ATTCCTA1550.0295.02
TTCCCTA800.0295.02
Produced by FastQC (version 0.11.5)