FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491958

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491958
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83029
Sequences flagged as poor quality0
Sequence length301
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACTAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG13121.5801707837020802No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA13081.5753531898493298No Hit
GGACTACTCGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG13021.5681267990702044No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA12751.5356080405641401No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA10751.294728347926628No Hit
GGACTACTAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG10611.2778667694420023No Hit
GGACTACTCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG10391.2513700032518758No Hit
GGACTACCCGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG9961.1995808693348107No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA9951.1983764708716231No Hit
GGACTACCAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG9801.1803104939238096No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA9571.1526093292704958No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA9171.1044333907429935No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA9021.08636741379518No Hit
GGACTACTGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG9001.083958616868805No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA8321.0020595213720507No Hit
GGACTACCCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG7980.9611099736236737No Hit
GGACTACCAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG7720.929795613580797No Hit
GGACTACTGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG7540.908116441243421No Hit
GGACTACACGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG7420.8936636596851703No Hit
GGACTACAAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG7270.8755976827373567No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA7130.858736104252731No Hit
GGACTACCGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG7070.8515097134736057No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6980.8406701273049176No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6790.817786556504354No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6740.811764564188416No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6300.7587710318081634No Hit
GGACTACACGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG6060.729865468691662No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5910.7117994917438485No Hit
GGACTACAAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG5800.6985511086487854No Hit
GGACTACCGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG5480.6600103578267834No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5400.650375170121283No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5340.6431487793421575No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4130.49741656529646266No Hit
GGACTACAGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG4120.4962121668332751No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC4030.48537258066458705No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC3870.4661022052535861No Hit
GGACTACAGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG3570.4299702513579593No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3160.3805899143672693No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC3150.3793855159040817No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC3080.37095472666176876No Hit
GGACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2840.3420491635452673No Hit
GGACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2760.33241397583976684No Hit
GGACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2700.3251875850606415No Hit
GGACTACCAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2440.2938732250177649No Hit
GGACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2390.28785123270182705No Hit
GGACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2390.28785123270182705No Hit
GGACTACCCGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2210.26617206036445096No Hit
GGACTACTGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2060.24810608341663756No Hit
GGACTACTAGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA2060.24810608341663756No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2050.24690168495345002No Hit
GGACTACTCGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA2040.24569728649026243No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC2040.24569728649026243No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1980.23847089571113708No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1910.23004010646882414No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1710.2059521372050729No Hit
GGACTACTAGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1710.2059521372050729No Hit
GGACTACCGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1700.20474773874188537No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1690.2035433402786978No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1670.20113454335232267No Hit
GGACTACTCGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1610.19390815257319732No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1590.1914993556468222No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1560.18788616025725952No Hit
GGACTACTCGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1510.1818641679413217No Hit
GGACTACCAGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1480.17825097255175903No Hit
GGACTACTAGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1470.17704657408857147No Hit
GGACTACCAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1460.1758421756253839No Hit
GGACTACTGGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1400.16861578484625853No Hit
GGACTACACGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1360.16379819099350829No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1340.16138939406713318No Hit
GGACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1340.16138939406713318No Hit
GGACTACCCGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1340.16138939406713318No Hit
GGACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1300.1565718002143829No Hit
GGACTACTAGGGTATCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG1290.15536740175119537No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1290.15536740175119537No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1270.15295860482482024No Hit
GGACTACTGGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1220.14693661250888246No Hit
GGACTACTAGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1220.14693661250888246No Hit
GGACTACTCGGGTATCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG1220.14693661250888246No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1200.14452781558250732No Hit
GGACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1200.14452781558250732No Hit
GGACTACCCGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1190.14332341711931976No Hit
GGACTACAGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1180.1421190186561322No Hit
GGACTACTAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1150.13850582326656952No Hit
GGACTACCCGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1140.13730142480338195No Hit
GGACTACTCGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1140.13730142480338195No Hit
GGACTACCAGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1130.13609702634019438No Hit
GGACTACACGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1130.13609702634019438No Hit
GGACTACTCGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1120.13489262787700684No Hit
GGACTACCCGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1110.13368822941381928No Hit
GGACTACCCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1110.13368822941381928No Hit
GGACTACTCGGGTTTCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG1090.13127943248744414No Hit
GGACTACCAGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1090.13127943248744414No Hit
GGACTACCAGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1080.1300750340242566No Hit
GGACTACTAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1080.1300750340242566No Hit
GGACTACCGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1080.1300750340242566No Hit
GGACTACAAGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1060.12766623709788144No Hit
GGACTACCAGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1060.12766623709788144No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1060.12766623709788144No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1060.12766623709788144No Hit
GGACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1050.1264618386346939No Hit
GGACTACTGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1040.12525744017150633No Hit
GGACTACTGGGGTATCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG1030.12405304170831878No Hit
GGACTACCGGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1030.12405304170831878No Hit
GGACTACTAGGGTTTCTAACTGAAGGGGCGATTTCCCTTCACATGTCCAC1010.12164424478194366No Hit
GGACTACCCGGGTTTCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG990.11923544785556854No Hit
GGACTACCGGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC990.11923544785556854No Hit
GGACTACTGGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC980.11803104939238097No Hit
GGACTACTGGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC980.11803104939238097No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC970.11682665092919342No Hit
GGACTACTGGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC970.11682665092919342No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC960.11562225246600585No Hit
GGACTACTGGGGTATCTAACTGAAGGGGCGATTTCCCTTCACATGTCCAC960.11562225246600585No Hit
GGACTACTCGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT960.11562225246600585No Hit
GGACTACTGGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC960.11562225246600585No Hit
GGACTACCCGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT960.11562225246600585No Hit
GGACTACCAGGGTATCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC950.1144178540028183No Hit
GGACTACCCGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC950.1144178540028183No Hit
GGACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC940.11321345553963075No Hit
GGACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC940.11321345553963075No Hit
GGACTACTAGGGTTTCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG940.11321345553963075No Hit
GGACTACCCGGGTATCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG940.11321345553963075No Hit
GGACTACTAGGGTATCTAACTGAAGGGGCGATTTCCCTTCACATGTCCAC930.11200905707644317No Hit
GGACTACTCGGGTTTCTAACTGAAGGGGCGATTTCCCTTCACATGTCCAC910.10960026015006805No Hit
GGACTACCAGGGTATCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG910.10960026015006805No Hit
GGACTACTCGGGTATCTAACTGAAGGGGCGATTTCCCTTCACATGTCCAC880.10598706476050536No Hit
GGACTACCCGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC860.10357826783413025No Hit
GGACTACACGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA850.10237386937094269No Hit
GGACTACCCGGGTATCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC840.10116947090775513No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGATCG108.4510114E-4294.9988295
GGACTAC79650.0294.6131
ACTCGGG12650.0291.500766
CTCGGGT12600.0291.48697
ACGGGTA3850.0291.16768
CAAGGGT7550.0291.091527
CACGGGT7400.0291.01237
TACTCGG12700.0290.353155
AGGGTAT18000.0290.082159
ACTGGGG8500.0289.792946
CAGGGTA5500.0289.63528
CGGGTAT16450.0289.618879
GACTACC26900.0289.515532
CTACCCG10250.0289.24274
ACCCGGG10200.0289.21456
ACAAGGG7600.0289.176456
ACACGGG7450.0289.05926
CTACCGG7200.0288.8534
ACCGGGG7000.0288.67746
AAGGGTA4600.0288.58588
Produced by FastQC (version 0.11.5)